多烯紫杉醇石墨烯的制备及质量评价

目的： 合成新型的多烯紫杉醇石墨烯复合材料（Fe₃O₄/GO-NA/DOC），并对其进行质量评价。方法： 利用透射电镜观察其形貌；以高效液相色谱法（HPLC）测定多烯紫杉醇（DOC）的含量，并对生成物的包封率、回收率以及精密度进行评价，以低温高速离心法测定Fe₃O₄/GO-NA/DOC复合材料的包封率。结果： 多烯紫杉醇检测浓度线性范围为20~120 μg·mL^-1（r=0.999 1），平均回收率（n=3）为100.2%±0.23%，在转速为20 000 r·min^-1，离心时间为80 min的条件下，包封率的值在90%以上。结论： Fe₃O₄/GO-NA/DOC复合材料制备工艺可行，质量控制方法简便可靠。

Objective: To synthetize the new-type Fe₃O₄/GO-NA/DOC composite materials,and evaluate their qualities. Methods: The morphology was observed by transmission electron microscope. The concentration of DOC was determined by high efficiency liquid chromatography. The recovery,precision and entrapment efficiency of Fe₃O₄/GO-NA/DOC composite materials were studied,and the Fe₃O₄/GO-NA/DOC composite materials entrapment efficiency was determined by Low temperature high speed centrifugation. Results: The linear detection concentration range of DOC was 20-120 μg·mL^-1 (r=0.999 1) with the average recovery (n=3) of 100.2%±0.23%. At a speed of 20 000 r·min^-1 and a centrifugation time of 80 min,the encapsulation rate was more than 90%. Conclusion: The preparation technique of Fe₃O₄/GO-NA/DOC composite materials is feasible,and the quality control method is convenient and reliable.

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