HPLC-DAD-ESI-MSⁿ分析双黄连注射液中22个化学成分

目的： 采用高效液相色谱（HPLC）-DAD-ESI-MSⁿ联用技术分析双黄连注射液中化学成分。方法： 采用Phenomenex Luna C₁₈色谱柱（250 mm×4.60 mm，5 μm），以甲醇-10 mmol·L^-1乙酸铵为流动相，梯度洗脱，流速1.0 mL·min^-1，检测波长254 nm，柱温30℃；采用电喷雾离子源（ESI）正负离子模式检测，质谱扫描m/z 100~1 500。对双黄连注射液的氯仿、乙酸乙酯、正丁醇和水4个溶剂萃取部位的化学成分进行分析。结果： 利用多级质谱信息，结合对照品对照及文献数据分析，初步确定了注射液中22个成分的结构，包括3种糖类成分，3种奎宁酸类成分，4种苯乙醇苷类成分，6种木脂素类成分，3种黄酮苷类成分，1种三萜皂苷类成分和2种其他类成分。结论： HPLC-DAD-ESI-MSⁿ联用技术对于中药注射液物质基础的研究有较好的应用价值。

Objective: To analyze the chemical constituents of Shuanghuanglian injection by HPLC-DAD-ESI-MSⁿ.Methods: The chromatography separation was performed on a Phenomenex Luna C₁₈ column(250 mm×4.60 mm,5 μm)maintained at 30℃.Methanol and 10 mmol·L^-1 ammonium acetate were used as the mobile phase in gradient elution at a flow rate of 1.0 mL·min^-1. Electrospray ionization source was used and the MS scanning was set at the range of m/z 100-1 500. The components in the extrations of chloroform, ethyl acetate, n-butanol and water in Shuanghuanglian injection were detected at 254 nm and positive and negative mode.Results: Based elution at a flow rate of 1.0 mL·min^-1 on their MSⁿ fragmentation pattern information,22 compounds including three carbohydrates, three quinic acids, four phenylethanoids, six lignans, three flavonoids, one saponin and two other ingredients were primarily identified by comparing with reference standards and reference data.Conclusion: HPLC-DAD-ESI-MSⁿ technology was applicable for the study of material base of traditional Chinese medicine injection.

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