HPLC-DAD双波长法同时测定小秦艽花中5个成分的含量

目的： 建立HPLC法测定蒙药小秦艽花中大麦黄苷、马钱苷、肥皂草苷、异荭草苷和异牡荆苷的含量。方法： 采用Symmetry? C₁₈色谱柱（4.6 mm×250 mm，5.0 μm），以乙腈（A）-0.1%甲酸水溶液（B）为流动相，梯度洗脱，流速为1.0 mL· min^-1，柱温35℃，检测波长为245 nm和330 nm。结果： 大麦黄苷、马钱苷、肥皂草苷、异荭草苷和异牡荆苷5个成分的进样量分别在0.124~2.484 μg（r=0.999 3）、0.127~2.540 μg（r=1.000）、0.015~0.302 μg（r=0.999 9）、0.112~2.396 μg（r=1.000）、0.024~0.482 μg（r=0.999 7）范围内线性关系良好；平均回收率（n=3）在96.5%~102.8%范围内，RSD为0.88%~2.8%。16批小秦艽花样品中大麦黄苷、马钱苷、肥皂草苷、异荭草苷和异牡荆苷的含量范围分别为1.15~7.71、0.38~9.23、0.13~0.86、1.11~7.46、0.59~1.70 mg·g^-1。结论： 该方法可以用于小秦艽花中5个活性成分的含量测定，为小秦艽花药材的资源开发提供科学依据。

Objective: To establish an HPLC method for simultaneous determination of lutonarin,loganin,saponarin,isoorientin and isovitexin in flowers of Gentiana dahurica. Methods: The analysis was performed on Symmetry? C₁₈ column (4.6 mm×250 mm,5.0 μm) with acetonitrile (A)-0.1% formic acid(B) as mobile phase with gradient elution. The flow rate was 1.0 mL·min^-1,the column temperature was 35℃,and the detection wavelengths were 245 nm and 330 nm. Results: There were good linearity in the ranges of 0.124-2.484 μg (r=0.999 3) for lutonarin,0.127-2.540 μg (r=1.000) for loganin,0.015-0.302 μg (r=0.999 9) for saponarin,0.112-2.396 μg (r=1.000) for isoorientin and 0.024-0.482 μg (r=0.999 7) for isovitexin. The average recoveries (n=3) of five components were in the range of 96.5%-102.8%,and the RSDs were in the range of 0.88%-2.8%. The content ranges of lutonarin,loganin,saponarin,isoorientin,isovitexin in the 16 batches of flowers of G. dahurica were 1.15-7.71 mg·g^-1,0.38-9.23 mg·g^-1,0.13-0.86 mg·g^-1,1.11-7.46 mg·g^-1 and 0.59-1.70 mg·g^-1,respectively. Conclusion: The method can be used for the determination of five index chemical components in flowers of G. dahurica,providing a scientific basis for the resource development of flowers of G. dahurica.

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