HPLC法同时测定真菌竹黄胶囊中3个醌类成分的含量

目的： 建立同时测定真菌竹黄胶囊中竹红菌丙素、竹红菌甲素、竹红菌乙素3个苝醌类成分含量的高效液相色谱法。方法： 采用CAPCELL PAK C₁₈色谱柱（250 mm×4.6 mm，5 μm），以乙腈-0.1%磷酸溶液（62：38）为流动相，流速1.0 mL·min^-1，检测波长460 nm，柱温30℃。结果： 竹红菌丙素、竹红菌甲素、竹红菌乙素进样量分别在0.016~0.816 μg（r=0.999 9）、0.020~0.982 μg（r=0.999 9）、0.040~2.016 μg（r=0.999 9）范围内与峰面积呈现良好的线性关系；平均回收率（n=3）分别为98.4%~98.6%（RSD<1.2%）、100.4%~100.8%（RSD<0.6%）、97.9%~100.2%（RSD<1.0%）；3批样品中竹红菌丙素、竹红菌甲素、竹红菌乙素含量范围分别为0.434~0.441、0.661~0.674、1.308~1.323 mg·粒^-1。结论： 该方法可用于真菌竹黄胶囊中竹红菌丙素、竹红菌甲素、竹红菌乙素的含量测定。

Objective: To establish an HPLC method for the simultaneous determination of three perylenequinones(hypocrellin C,hypocrellin A and hypocrellin B)in Zhenjun Zhuhuang capsules. Methods: The CAPCELL PAK C₁₈(250 mm×4.6 mm,5 μm)was used with the mobile phase of acetonitrile-0.1% phosphoric acid solution(62:38)at a flow rate of 1.0 mL·min^-1. The detection wavelength was 460 nm and the column temperature was 30℃. Results: The linear ranges of hypocrellin C,hypocrellin A and hypocrellin B were 0.016-0.816 μg(r=0.999 9), 0.020-0.982 μg(r=0.999 9)and 0.040-2.016 μg(r=0.999 9),respectively. The average recoveries(n=3)were 98.4%-98.6%(RSD<1.2%),100.4%-100.8%(RSD<0.6%)and 97.9%-100.2%(RSD<1.0%),respectively. The contents of hypocrellin C,hypocrellin A and hypocrellin B in three batches of samples were 0.434-0.441,0.661-0.674,1.308-1.323 mg per capsule,respectively. Conclusion: The method can be used to determine contents of hypocrellin C,hypocrellin A and hypocrellin B in Zhenjun Zhuhuang capsules.

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