Objective: To develop an HPLC-TOF/MS method for the separation and identification of cis-trans isomers of retinyl esters in fish liver oil. Methods: The retinyl esters were separated by gradient elution with a mobile phase of MTBE-methanol-water on a YMC C30 (250 mm×4.6 mm,5 μm) column. The structures of retinyl esters in fish liver oil were elucidated by ESI+-TOF/MS and by comparing with reference substances. Results: Cod liver oil capsule contain all-trans and 13-cis retinyl palmitate. Shark liver oil contains all-trans retinyl oleate,retinyl palmitate,retinyl stearate,retinyl gondoate,retinyl heptadecanoate and 13-cis retinyl oleate,retinyl palmitate,retinyl stearate. Conclusion: The established method is useful for the separation and identification of all-trans and 13-cis retinyl esters in fish liver oil. The results obtained are valuable for its manufacturing process and quality control.
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