Objective: To study arsenic,soluble arsenic and the speciation of soluble arsenic in realgar and 5 kinds of compound preparations by AFS and HPLC-AFS, and provide reference for evaluation of quality and safety of realgar-containing drugs. Methods: After microwave digestion,the total arsenic content in samples was determined by atomic fluorescence spectrometry. After artificial gastric fluid oscillation extraction or methanol-water (1:1) ultrasonic extraction,determine the content of soluble total arsenic in samples by atomic fluorescence spectrophotometry. Analyze the speciation of arsenic and determine its content by high performance liquid phase-atomic fluorescence. Results: The linear relationship was good in the range of 0-10 μg·L-1 in AFS,The recovery of standard addition was 94.0% in arsenic,RSD 2.2%. The recovery of standard addition in soluble arsenic was 96.4%-96.5%,RSD 1.9%-7.7%. The linear relationship was good in the range of 0-100 μg·L-1 in HPLC-AFS. 84.9%-101.2% by methanol-water (1:1) ultrasonic extraction,RSD 2.5%-8.2%;83.2%-93.8% by artificial gastric fluid oscillation extraction,RSD 2.3%-7.2%. The content of total soluble arsenic in realgar and its compound preparations accounted for only 0.26% -1.05% of total arsenic in the sample. Arsenic species detected in all samples were As (III) and As (V) only. Conclusion: AFS and HPLC-AFS are suitable to the detection of the arsenic,soluble arsenic and speciation of soluble arsenic in realgar and 5 kinds of compound preparations.
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