HPLC法同时测定半夏中5个代表性成分的含量

目的：建立高效液相色谱法同时测定半夏药材中肌苷、鸟苷、腺苷、琥珀酸、盐酸麻黄碱5个代表性成分含量。方法：采用Agilent Eclipse XDB C₁₈色谱柱（4.6 mm×250 mm，5 μm）；以乙腈（A）-水（B）为流动相，梯度洗脱；流速1.0 mL·min^-1；检测波长265 nm；柱温30℃；进样量10 μL。结果：半夏样品中5个成分的色谱峰均达到基线分离，肌苷、鸟苷、腺苷、琥珀酸、盐酸麻黄碱的线性方程分别为Y=769.1X+3.364（r=0.999 7）、Y=826.3X-7.726（r=0.999 6）、Y=841.7X-0.700 6（r=0.999 3）、Y=253.3X+1.821（r=0.999 6）、Y=362.7X+1.530（r=0.999 3）；平均加样回收率分别为99.2%（RSD=1.1%）、101.4%（RSD=1.4%）、99.4%（RSD=1.0%）、99.3%（RSD=1.3%）、100.8%（RSD=0.91%）；10批半夏药材中5个成分含量分别为肌苷0.21~0.33 mg·g^-1、鸟苷2.02~2.18 mg·g^-1、腺苷2.42~2.70 mg·g^-1、琥珀酸3.24~4.43 mg·g^-1、盐酸麻黄碱5.89~6.74 mg·g^-1。结论：建立的半夏中5个代表性成分含量测定方法准确灵敏，稳定性和重复性好，可为半夏的质量控制提供研究方法。

Objective: To establish an HPLC method for the simultaneous determination of five representative components of inosine, guanosine, adenosine, succinic acid and ephedrine hydrochloride in Pinelliae Rhizoma.Method: Samples were analyzed on an Agilent Eclipse XDB C₁₈ column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile (A) and water (B) in a gradient elution.The flow rate of the mobile phase, detection wavelength, column temperature and injection volume were set at 1.0 mL·min^-1, 265 nm, 30℃ and 10 μL, respectively.Results: The chromatographic peaks of the five components in the Pinelliae Rhizoma samples were all separated at baseline.The linear equations of inosine, guanosine, adenosine, succinic acid and ephedrine hydrochloride were Y=769.1X+3.364 (r=0.999 7), Y=826.3X-7.726 (r=0.999 6), Y=841.7X-0.700 6 (r=0.999 3), Y=253.3X+1.821 (r=0.999 6) and Y=362.7X+1.530 (r=0.999 3), respectively;The recoveries were 99.2% (RSD=1.1%), 101.4% (RSD=1.4%), 99.4% (RSD=1.0%), 99.3% (RSD=1.3%), and 100.8% (RSD=0.91%), respectively.The contents of the above-mentioned five components in 10 batches of Pinelliae Rhizoma were 0.21-0.33 mg·g^-1, 2.02-2.18 mg·g^-1, 2.42-2.70 mg·g^-1, 3.24-4.43 mg·g^-1 and 5.89-6.74 mg·g^-1, respectively.Conclusion: The established method for determination of five representative components in Pinelliae Rhizoma is accurate, stable and reproducible, and can provide a research method for quality control of Pinelliae Rhizoma.

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