新疆一枝蒿不同极性部位体外抗炎作用的谱-效关系分析

目的：研究新疆一枝蒿不同极性部位指纹图谱与体外抗炎作用的相关性，为揭示其抗炎作用物质基础提供依据。方法：采用高效液相色谱法建立新疆一枝蒿不同极性提取物及洗脱物的指纹图谱；以巨噬细胞RAW264.7为研究对象，考察新疆一枝蒿不同极性提取物及洗脱物对细胞上清液中一氧化氮（NO）、肿瘤坏死因子（TNF-α）、白介素-6（IL-6）、白介素-10（IL-10）和转录因子（NF-κB）的抑制作用，比较其抗炎活性差异；采用灰色关联度分析法建立谱-效关系。结果：新疆一支蒿不同极性提取物及洗脱物的指纹图谱有明显差异性，从HPLC指纹图谱中确定18个共有峰。体外抗炎结果显示，不同极性提取物及洗脱部位的抗炎作用存在显著差异，其中50%乙醇提取物的30%洗脱部位对体外炎症因子的抑制作用最强。谱-效结果分析，对药效贡献较大的峰为5个共有峰，分别为1、2、5、9、11号。结论：建立了新疆一支蒿的HPLC指纹图谱及其体外抗炎活性的谱-效关系，并确定了抗炎活性的主要有效成分，为该药材的药效物质基础研究提供参考。

Objective:To study the correlation between fingerprints and the anti-inflammatory effect in vitro of different polar parts of Artemisia rupestris L.,so as to reveal the material base of efficacy. Methods:HPLC was constructed to establish fingerprints of different polar extracts and eluates from Artemisia rupestris L. Using macrophage RAW264.7 as object,the inhibitory effects against NO,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-10(IL-10)and transcription factors(NF-κB)were investigated and anti-inflammatory effects of different parts were compared. The grey correlation analysis method was used to establish the spectrum-activity relationship. Results:The fingerprint of different polar extracts and eluates from Artemisia rupestris L. showed significant difference. 18 common peaks were determined. In vitro anti-inflammatory results indicated that the anti-inflammatory effects of different polar extracts and eluates were significant different. Among them,30% eluate from 50% ethanol extract had the strongest effect. Analysis of spectrum-activity relationship showed that 5 components had greater contribution to the effect,the peaks ranked as No.1,2,5,9 and 11. Conclusion:The spectrum-activity relationship of fingerprint and anti-inflammatory effect in vitro are established and the main anti-inflammatory components are identified,which providing reference for its study on therapeutic material basis.

[1] 冯鑫,房德敏,周永梅.谱效关系分析在中药组方研究中的应用进展[J].中国中医基础医学杂志,2018,24(3):422 FENG X,FANG DM,ZHOU YM.Progress in the application of spectral activity relationship analysis in the study of TCM formulations[J].Chin J Basic Med Tradit Chin Med,2018,24(3):422
[2] 王琰,王虹熙.中药谱效关系的研究进展[J].中国民族民间医药,2018,27(4):50 WANG Y,WANG HX.Progress in the study of profile-effect of traditional Chinese medicine[J].Chin J Ethnmed Ethnopharm,2018,27(4):50
[3] 吉腾飞,杨建波,宋卫霞,等.新疆一枝蒿化学成分研究Ⅱ[J]. 中国中药杂志,2007,32(12):1187 JI TF,YANG JB,SONG WX,et al.Studies on the chemical constituents of the Artemimsia rupestris L.in Xinjiang[J].China J Chin Mater Med,2007,32(12):1187
[4] 王燕,吐尔洪·阿西木,堵年生.新疆一枝蒿化学成分的研究[J]. 新疆医科大学学报,2004,27(1):361 WANG Y,TU-ER-HONG·AXM,DU NS.Studies on the chemical constituents of the Artemisia rupestris L.in Xinjiang[J].J Xinjiang Med Univ,2004,27(1):361
[5] 万英洁,夏建新,唐丽.新疆一枝蒿化学成分、药理作用及临床应用研究进展[J].中国中药杂志,2017,42(23):4565 WAN YJ,XIA JX,TANG L.Chemical constituents,biological activities and clinical applications of Artemisia rupestris[J].China J Chin Mater Med,2017,42(23):4565
[6] 董慧君,蒋云涛,俞可云,等.维药新疆一枝蒿的研究进展[J]. 现代中药研究与实践,2013,27(4):73 DONG HJ,JIANG YT,YU KY,et al.Studies on the Artemimsia rupestris L.in Xinjiang[J].Chin Med J Res Pract,2013,27(4):73
[7] 蔡晓翠,贺金华,顾政一,等.HPLC-MS/MS法同时测定新疆一枝蒿药材中5个成分的含量[J].药物分析杂志,2013,33(10):1672 CAI XC,HE JH,GU ZY,et al.Simultaneous determination of 5 constituents in Artemisia rupestris L.by HPLC-MS/MS[J].Chin J Pharm Anal,2013,33(10):1672
[8] 蔡晓翠,顾政一,贺金华,等.新疆一枝蒿药材人工种植品种与野生品种的差异性分析[J].中国药房,2017,28(16):2224 CAI XC,GU ZY,HE JH, et al.Analysis of the differences between artificially cultivated and wild Xinjiang Artemisia rupestris[J].China Pharm,2017,28(16):2224
[9] 张洁,顾政一,何承辉.HPLC法同时测定新疆一枝蒿中木犀草素和一枝蒿酮酸的含量[J].中国实验方剂学杂志,2009,15(4):1 ZHANG J,GU ZY,HE CH.Determination of luteolin and rupestonic acid in Artemisia rupestris by high performance liquid chromatography[J].Chin J Exp Tradit Med Form,2009,15(4):1
[10] XU W,ZHOU Q,YAO Y,et al.Inhibitory effect of gardenblue blueberry(Vaccinium ashei Reade)anthocyanin extracts on lipopolysaccharide-stimulated inflammatory response in RAW 264.7 cells[J].J Zhejiang Univ Sci,2016,17(6):425
[11] 王书侠,张家明,姚孝明,等.木犀草素对活化的RAW 264.7巨噬细胞分泌炎症因子的影响[J].医学研究生学报,2017,30(1):31 WANG SX,ZHANG JM,YAO XM,et al.Effects of luteolin on the secretion of inflammatory cytokines from activated RAW264.7 macrophages[J].J Med Postgraaduates,2017,30(1):31
[12] 毛艳,贺金华,蔡晓翠,等.维药神香草提取物体外抗炎作用的谱效关系研究[J].中国药房,2017,28(10):1364 MAO Y,HE JH,CAI XC,et al.Study on the spectrum-effect relationship of anti-inflammation effect in vitro of extracts from Uygur medicine Hyssopus cuspidatus[J].China Pharm,2017,28(10):1364
[13] 秦子茹,贺金华,顾政一,等.一枝蒿不同极性提取物抗病毒作用的谱效关系研究[J].中国药房,2015,26(7):889 QIN ZR,HE JH,GU ZY,et al.Study on the spectrum-effect relationship of anti-virus effect of Artemisia rupestris extracts from different solvents[J].China Pharm,2015,26(7):889
[14] 许俊洁,卢金清,李肖爽,等.神农香菊挥发油体外抗氧化活性的谱效关系研究[J].药物分析杂志,2016,36(10):1823 XU JJ,LU JQ,LI XS,et al.Study on spectrum-effect relationship between fingerprints o f essential oil from Shennong Xiangju and the in vitro anti-oxidant activities[J].Chin J Pharm Anal,2016,36(10):1823
[15] 梁健钦,王剑,熊万娜,等.基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系[J].中国实验方剂学杂志,2015,21(1):121 LIANG JQ,WANG J,XIONG WN,et al.Spectrum-effect relationship of leaf extracts from Mangifera indica based on grey relational analysi[J].Chin J Exp Tradit Med Form,2015,21(1):121
[10] 刘思峰,党耀国,方志耕,等.灰色系统理论及其应用[M].第5版.北京:科学出版社,2010:62 LIU SF,DANG YG,FANG ZG,et al.Grey System Theory and Its Application[M].5th Ed.Beijing:Science Press,2010:62