基于主成分分析的前胡类药材HPLC特征图谱研究

目的：利用高效液相色谱技术，建立前胡及其易混用品紫花前胡和硬前胡的特征图谱，结合主成分分析，用于该类药材的鉴别和质量控制。方法：采用Aglient TC C₁₈色谱柱（4.5 mm×250 mm，5 μm），以水（A）-甲醇（B）为流动相，梯度洗脱（0~15 min，40% B；15~16 min，40% B→48% B；16~26 min，48% B→75% B；26~55 min，75% B→95% B；55~56 min，95% B→99% B；56~60 min，99% B，流速1.0 mL·min^-1，检测波长325 nm，柱温30℃，进样量5 μL，分别建立不同产地前胡、紫花前胡、硬前胡的HPLC特征图谱，并通过Chempattern及Ezinfo化学计量学软件对所采集的数据进行主成分分析。结果：主成分分析结果显示前胡及其易混用品分别聚在不同的区域，所含化学成分存在明显差异。结论：本方法稳定性、重现性好，所建立的特征图谱专属性强，可用于前胡及其易混用品的鉴别和质量控制。

Objective:To establish HPLC characteristic fingerprints of Peucedani Radix and its promiscuous herbs(Peucedani Decursivi Radix and Peucedani Subglabri Radix),and to identify and control the quality of the medicinal materials by principle component analysis(PCA). Methods:The HPLC analysis was carried out on an Aglient TC-C₁₈ column(4.6 mm×250 mm,5 μm)with water(A)-methanol(B)as mobile phase by gradient elution(0-15 min,40%B;15-16 min,40%B→48%B;16-26 min,48%B→75%B;26-55 min,75%B→95%B;55-56 min,95%B→99%B;56-60 min,99%B),at a flow rate of 1.0 mL·min^-1. The column temperature was set at 30℃,the detection wavelength was set at 325 nm,and the injection volume was 5 μL. HPLC characteristic fingerprints of Peucedani Radix,Peucedani Decursivi Radix and Peucedani Subglabri Radix from different habitats were established,respectively. The obtained data were analyzed by Chempattern and Ezinfo chemometrics software. Results:PCA were used to analyze the LC-MS data,the results showed that Peucedani Radix,Peucedani Decursivi Radix and Peucedani Subglabri Radix clustered in different zones respectively,and chemical compositions in Peucedani Radix and its promiscuous herbs varied obviously. Conclusion:The proposed method is stable and reproducible,and the established characteristic fingerprint is specific,which can be used for identification and quality control of Peucedani Radix and its promiscuous herbs.

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