Objective:To establish an HPLC method for simultaneous determination of 5 components(gallic acid,berberine hydrochloride,forsythiaside A,rutin and quercetin)in Ningmitai capsules. Methods:The separation was performed on a Diamonsil C18 column(250 mm×4.6 mm,5 μm),with methanol-0.1% phosphoric acid as the mobile phase in gradient elution at a flow rate of 1.0 mL·min-1. The column temperature was 25℃ and the detection wavelengths were 256,280 and 330 nm. Results:The linear relationship of gallic acid in 0.004 9-0.121 5 mg·mL-1, berberine at 0.028 4-0.710 0 mg·mL-1,forsythione A at 0.227 0-5.675 0 mg·mL-1,rutin at 0.014 4-0.360 5 mg·mL-1 and quercetin at 0.062 2-1.554 0 mg·mL-1 were all good(r>0.999 5). The average recoveries were 98.1%-99.5% and the RSDs were 0.81%-1.8%,respectively. The average contents of gallic acid,berberine,forsythin A,rutin and quercetin in Ningmitai capsules samples were 0.022%,0.078%,0.552%,0.040% and 0.163%,respectively. Conclusion:This method is simple,accurate and reproducible,and can be used for the quality control of Ningmitai capsules.
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