蒙药铁杆蒿中12个指标成分的同时测定

目的：建立铁杆蒿中12个指标成分的含量测定方法。方法：采用高效液相色谱法对不同来源铁杆蒿进行含量测定，色谱条件：色谱柱为Inert Sustain C₁₈ （250 mm×4.6 mm，5 μm），以乙腈-水为流动相梯度洗脱，检测波长为265 nm，柱温为30℃。结果：在选用的实验色谱条件下，S1~S12的校准曲线表现出良好的线性关系（r>0.999 3），平均回收率在93.3%~98.7%范围内，RSD均小于3.0%。不同来源样品中12个指标成分的含量随产地的不同而有显著差异。结论：通过实验研究，建立了铁杆蒿中12个指标成分的含量测定方法，为综合评价该药材质量提供依据。

Objective:To develop a method for simultaneous determination of 12 components in the aerial parts of Artemisa sacrorum Ledeb.(A.Sacrorum)Methods:The aerial parts of A.sacrorum collected from different regions were determined by HPLC.The separation was performed on an Inert Sustain C₁₈ column (250 mm×4.6 mm,5 μm) at 30℃ with methanol-water as the mobile phase by gradient elution.The detection wavelength was 261 nm.Results:Under the chromatographic conditions adopted in this study,all calibration curves exhibited good linearity (r > 0.999 3) in a relatively wide concentration range.The recoveries were within the range of 93.3%-98.7% with RSDs less than 3%.The contents of all compounds (S1-S12) in different samples varied significantly with the different habitats.Conclusion:The quantitative method for evaluating the quality of the aerial parts of A.sacrorum is established,which an be used for comprehensive quality evalustion of the herb.

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