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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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粪便中短链脂肪酸测定方法研究进展

Advance in determination of short-chain fatty acids of gut bacterial metabolites in feces

作者(英文):
分类号:R917
出版年·卷·期(页码):2019,39 (6):967-974
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

肠道菌代谢产物如短链脂肪酸在机体生理和病理过程中扮演着重要角色。粪便中短链脂肪酸的测定是研究肠道菌群结构和功能的重要研究手段之一。短链脂肪酸具有挥发性,且基质复杂,增加了粪便中短链脂肪酸分析的难度。本文对近年来粪便中短链脂肪酸测定方法的研究进展进行综述,重点对样品除杂、衍生化等样品预处理方法及分析中涉及的色谱方法、色谱柱和检测器等进行总结,为进一步开发利用高效和灵敏的粪便短链脂肪酸分析方法,提升肠道菌群功能科学研究水平提供参考。

-----英文摘要:---------------------------------------------------------------------------------------

The metabolites such as short-chain fatty acids (SCFAs) of gut microbiota are important roles engaged in gut microbiota related physiological and pathological processes. The composition and function of gut microbiota are currently one of hot topics in life science. Determination of SCFAs in feces is one of the important techniques for investigating the composition and function of gut microbiota. As the complex matrix of feces,as well as the volatility characters of SCFAs,it increased the difficulty of analyzing short-chain fatty acids in feces.. In this paper,the recent achievements on the determination of SCFAs in feces were reviewed,in particular on the sample pretreatment,including extraction and derivatization,and the analytical method,chromatographic columns,detectors,etc,so as to provide references for development and application of more efficient and sensitive methods for determination of SCFAs,and thereby promote the comprehensive researches on composition and function of gut microbiota.

-----参考文献:---------------------------------------------------------------------------------------

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