HPLC-DAD法同时测定小儿退热合剂中多指标成分含量

目的：建立同时测定小儿退热合剂中8个成分的方法。方法：采用DIKMA Platisil ODS色谱柱（250 mm×4.6 mm，5 μm），柱温35℃，流动相为乙腈-0.1%磷酸水溶液，采用梯度洗脱，变换波长检测，分别为240 nm（京尼平苷酸、京尼平龙胆双糖苷、京尼平苷）、274 nm（绿原酸、黄芩苷、丹皮酚）和210 nm（连翘苷、柴胡皂苷A），流速为1 mL·min^-1。结果：京尼平苷酸、京尼平龙胆双糖苷、京尼平苷、绿原酸、黄芩苷、丹皮酚、连翘苷、柴胡皂苷A的线性范围分别为1.17~9.36 ng·mL^-1（r=0.999 5），1.98~15.84 ng·mL^-1（r=0.999 7），8.00-64.00 ng·mL^-1（r=0.999 9），1.64~13.12 ng·mL^-1（r=0.999 8），7.00~56.00 ng·mL^-1（r=0.999 9），1.096~8.768 ng·mL^-1（r=0.999 9），0.56~4.48 ng·mL^-1（r=0.999 9），6.20~49.60 ng·mL^-1（r=0.999 8），平均回收率在95.2~103.0%之间。3批样品中上述8个成分的含量测定结果分别为0.124 4~0.125 2、0.172 4~0.172 9、1.331 4~1.331 9、0.129 2~0.130 3、1.543 3~1.544 1、0.107 3~0.107 6、0.304~0.308、0.373 0~0.374 5 mg·mL^-1。结论：该法检测简便，稳定，可靠，可用于小儿退热合剂质量控制。

Objective:To establish a method for simultaneous determination of eight components in Xiaoer Tuire mixture.Methods:The separation was performed on a DIKMA Platisil ODS column (250 mm×4. 6 mm, 5 μm), column temperature was 35℃, acetonitriles and 0.1% H₃PO₄ aqueous solution was used as mobile phase for gradient elution.Detection wavelengths were 240 nm for geniposidic acid, genipin 1-gentiobioside, geniposide, 274 nm for chlorogenic acid, baicalin, paeonol and 210 nm for phillyroside, saikosaponin A, respectively. The flow rate was 1 mL·min^-1.Results:Linear ranges of geniposidic acid, genipin 1-gentiobioside, geniposide, chlorogenic acid, baicalin, paeonol, phillyroside, saikosaponin A concentrations were 1.17-9.36 ng·mL^-1 (r=0.999 5), 1.98-15.84 ng·mL^-1 (r=0.999 7), 8.00-64.00 ng·mL^-1 (r=0.999 9), 1.64-13.12 ng·mL^-1 (r=0.999 8), 7.00-56.00 ng·mL^-1 (r=0.999 9), 1.096-8.768 ng·mL^-1 (r=0.999 9), 0.56-4.48 ng·mL^-1 (r=0.999 9), 6.20-49.60 ng·mL^-1 (r=0.999 8), respectively. Their average recovery rates fell between 95.21% and 103.02%. The contents of the eight components in 3 batches of samples were 0.124 4-0.125 2, 0.172 4-0.172 9, 1.331 4-1.331 9, 0.129 2-0.130 3, 1.543 3-1.544 1, 0.107 3-0.107 6, 0.304-0.308, 0.373 0-0.374 5 mg·mL^-1. Conclusion:The method established is simple, stable, reliable and can be used as an effective method for quality control of Xiaoer Tuire mixture.

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