HPLC法同时测定藏药双花千里光中6个化学成分的含量

目的：建立同时测定藏药双花千里光中的绿原酸、芦丁、金丝桃苷、异槲皮苷、烟花苷和紫云英苷6个化学成分含量的HPLC方法。方法：双花千里光药材用70%乙醇-水提取；以乙腈（A）-0.1%甲酸水溶液（B）为流动相，梯度洗脱（0~2 min，0→16% A；2~6 min，16% A→15% A；6~8 min，15% A；8~13 min，15% A→20% A；13~16 min，20% A→25% A；16~19 min，25% A→20% A；19~26 min，20% A→30% A），检测波长为360 nm。结果：所测6个化学成分在所取质量浓度范围内的线性关系均良好，r值都大于0.999 0；精密度、重复性和稳定性良好；平均回收率为96.1%~99.4%，RSD为0.84%~1.8%；6个化学成分绿原酸、芦丁、金丝桃苷、异槲皮苷、烟花苷、紫云英苷的含量测定结果分别为1.75%、1.26%、0.86%、1.20%、0.57%、1.29%。结论：建立的HPLC方法可用于同时测定双花千里光药材中6个化学成分，可为藏药双花千里光药材的质量控制提供一定的参考。

Objective:To establish an HPLC method for simultaneous determination of six chemical components including chlorogenic acid, rutin, hyperoside, isoquercitrin, kaempferol-3-O-rutinoside, and astragalin in Tibetan medicine Senecio dianthus Franch..Methods:The Senecio dianthus Franch.herbal medicine was extracted with 70% ethanol-water.The mobile phase was acetonitrile (A) -0.1% aqueous formic acid (B) in gradient elution (0-2 min, 0→16%A;2-6 min, 16%A→15%A;6-8 min, 15%A;8-13 min, 15%A→20%A;13-16 min, 20%A→25%A;16-19 min, 25%A→20%A;19-26 min, 20%A→30%A).The detection wavelength was 360 nm.Results:The linear relationship of the six chemical components was good, and the r values were all above 0.999 0.Results of precision, repeatability and stability tests were good.The average recovery were 96.1%-99.4% with RSDs of 0.84%-1.8%.The contents of the six chemical components were 1.75% (chlorogenic acid), 1.26% (rutin), 0.86% (hyperoside), 1.20% (isoquercitrin), 0.57% (kaempferol-3-O-rutinoside), and 1.29% (astragalin).Conclusion:The established HPLC method can be used to simultaneously determine six chemical components in Senecio dianthus Franch..It can provide reference for the quality control of Tibetan medicine Senecio dianthus Franch..

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