Objective: To explore the effects of high-fat diet on intestinal microflora and insulin resistance, and the role of intestinal flora in the occurrence and development in the pre-diabetes diabetic rats. Methods: Rats were fed with high-fat diet to construct a high-fat diet-induced obesity group,the control group was fed normal diet. The blood and feces of rats were collected and the dynamic changes of fasting blood glucose,serum insulin,fecal free ammonia,short chain fatty acid and intestinal flora were measured in two groups. Results: The fasting blood glucose level of the model group was significantly higher than that of the control group at the 6th week (P<0.05). Compared with the control group,the serum insulin levels of the model group were significantly increased at the 3rd and 6th weeks (P<0.05),and the fecal free ammonia levels of the model group were significantly increased at the 3rd,6th and 9th weeks (P<0.05). The levels of acetic acid,propionic acid,butyric acid and total short chain fatty acids in the feces of the model group were significantly lower than those of the control group at the 9th week (P<0.05). The results of intestinal microflora diversity showed that there were significant differences in intestinal flora between the model group and the control group at the 9th week. Conclusion: High-fat diet can lead to unhealthy metabolic disorders in rats,including the high blood sugar, insulin resistance,fecal free ammonia levels,fecal short-chain fatty acid metabolism and intestinal flora structural disorders,thereby increasing the incidence of type Ⅱ diabetes mellitus process.
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