紫外分光光度法用于青霉素酶活力测定的研究

目的：建立紫外分光光度法测定青霉素酶活力，并研究中国药典青霉素酶活力单位与国际单位之间的关系。方法：利用青霉素与青霉噻唑酸在232 nm处的吸光系数不同，分别在30℃和37℃下，于232 nm处用紫外分光光度计监控青霉素酶与一定量青霉素的反应过程，通过反应时间计算青霉素酶的活力；同时用中国药典的碘量法进行测定。结果：本文所用的紫外分光光度法测定结果与中国药典规定的碘量法相比无显著性差异（=0.302>0.05）。中国药典青霉素酶活力单位（U）与国际单位（IU）的换算关系为：1 U=4.4×10⁷ IU+2.6×10⁵。结论：紫外分光光度法测定青霉素酶活力方法重复性好，操作简单，快速。建立的中国药典青霉素酶活力单位与国际单位之间的关系使两者具有可比性。

Objective: To establish a measuring method of penicillinase activity by UV spectrophotometry and to explore the relationship between penicillinase activity unit in Chinese pharmacopoeia and that in international standard.Methods: Since the absorbances of penicillinaces and penicillanic acid were different at 232 nm, the reaction between penicillinace and penicillinase was able to be monitored by ultraviolet spectrophotometer at 30℃ and 37℃.The value of penicillinase activity was calculated through the reaction time, and then compared with that of iodometric assay in ChP.Results: The results of UV spectrophotometry and iodimetric method had no significant difference(p=0.302>0.05).The relationship of Chinese pharmacopoeia unit of penicillinase activity(U) and international unit(IU)was 1 U=4.4×10⁷ IU+2.6×10⁵.Conclusion: The UV spectrophotometric method was simple, repeatable and rapid.It was comparable between penicillinase activity unit in Chinese pharmacopoeia and that in international standard.

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