Objective: To analyze the characteristic flavonoids in citrus, canola flower and milk vetch honeys.Methods: The flavonoids in canola flower honey, milk vetch honey, citrus honey and their nectar flowers were simultaneously analyzed by UPLC-MS/MS.The samples were analyzed on an Inertsil ODS-3 column(2.1 mm×75 mm, 2 μm),eluted with methanol-0.02% formic acid solution as mobile phase in gradient mode at a flow rate of 0.4 mL·min-1,the column temperature was controlled at 40℃ and the injection volume was 2 μL;electrospray ionization(ESI)source was applied and operated in the negtive or positive multiple reaction monitoring(MRM) mode, 16 flavonoids were used as reference, such as catechin, epicatechin, rutin, narirutin, morin, myricetin, kaempferol, pinocembrin etc.Results: The characteristic components and their contents(from highest to lowest) were different in different nectar honey:characteristic components in citrus honey were naringenin, luteolin, and apigenin wogonin; canola flower honey had naringenin, quercetin and Wogonin; and milk vetch honey had quercetin, kaempferol, apigenin and wogonin.Conclusion: The method with flowers as a "control" to find honey's characteristic component is sensitive, reliable, and traceable.The developed method was helpful to analyze the chemical components in flower nectar.
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