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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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UPLC-MS/MS法分析3种蜂蜜及其蜜源花中的特征黄酮类组分

Analysis of characteristic flavonoids in three kinds of honey and nectar flowers by UPLC-MS/MS

作者: 程巧鸳, 王笑笑 
作者(英文):
分类号:R917
出版年·卷·期(页码):2017,37 (6):994-1000
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的:分析柑橘、油菜和紫云英蜜中的特征黄酮类组分。方法:采用UPLC-MS/MS同时分析油菜蜜、紫云英蜜和柑橘蜜及其蜜源花中的黄酮类组分。色谱柱采用Inertsil ODS-3柱(2.1 mm×75 mm,2 μm),柱温40℃,以甲醇-0.02%甲酸为流动相梯度洗脱,流速为0.4 mL·min-1;以儿茶酸、表儿茶酸、芦丁、芸香柚皮苷、桑黄素、杨梅素、山柰酚、生松素等16个黄酮类对照品作为对照,采用电喷雾离子源(ESI),多反应监测(MRM)进行分析。结果:不同蜜源蜂蜜中的特征黄酮类组分及其含量(从高到低排列)有所不同:柑橘蜜的特征组分有柚皮素、木犀草素、芹菜素和汉黄芩素;油菜花蜜的特征组分有柚皮素、槲皮素和汉黄芩素;紫云英蜜的特征组分有槲皮素、山柰酚、芹菜素和汉黄芩素。结论:该方法灵敏度高,以蜜源花作为“对照”寻找蜂蜜中的特征成分具有数据可靠、可溯源性,并对蜜源花中化学成分的分析具有指导意义。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To analyze the characteristic flavonoids in citrus, canola flower and milk vetch honeys.Methods: The flavonoids in canola flower honey, milk vetch honey, citrus honey and their nectar flowers were simultaneously analyzed by UPLC-MS/MS.The samples were analyzed on an Inertsil ODS-3 column(2.1 mm×75 mm, 2 μm),eluted with methanol-0.02% formic acid solution as mobile phase in gradient mode at a flow rate of 0.4 mL·min-1,the column temperature was controlled at 40℃ and the injection volume was 2 μL;electrospray ionization(ESI)source was applied and operated in the negtive or positive multiple reaction monitoring(MRM) mode, 16 flavonoids were used as reference, such as catechin, epicatechin, rutin, narirutin, morin, myricetin, kaempferol, pinocembrin etc.Results: The characteristic components and their contents(from highest to lowest) were different in different nectar honey:characteristic components in citrus honey were naringenin, luteolin, and apigenin wogonin; canola flower honey had naringenin, quercetin and Wogonin; and milk vetch honey had quercetin, kaempferol, apigenin and wogonin.Conclusion: The method with flowers as a "control" to find honey's characteristic component is sensitive, reliable, and traceable.The developed method was helpful to analyze the chemical components in flower nectar.

-----参考文献:---------------------------------------------------------------------------------------

[1] 曹炜,尉亚辉.蜂产品保健原理与加工技术[M]. 北京:化学工业出版社, 2002:198 CAO W,WEI YJ. Bee Health Principles and Processing Technology[M]. Beijing:Chemical Industry Press,2002:198
[2] RAMANAUSKIENE K,STELMAKIENE A,BRIEDIS V,et al. The quantitative analysis of biologically active compounds in Lithuanian honey[J]. J Agric Food Chem,2012, 132(3):1544
[3] 王锦梅,程妮,丰凡,等.单花种蜂蜜植物源黄酮标记物的研究进展[J].食品与发酵工业, 2013, 39(1):161 WANG JM, CHENG N, FENG F, et al. Research of flavonoids as botanical origin markers of monofloral honey[J]. Food Ferment Ind, 2013, 39(1):161
[4] MARTOS I, FERRERES F,TOMÁS-BARBERÁN FA. Identification of flavonoid markers for the botanical origin of Eucalyptus honey[J]. J Agric Food Chem,2000, 48(5):1498
[5] MARTOS I, FERRERES F, YAO LH, et al. Flavonoids in monospecific Eucalyptus honeys from Australia[J]. J Agric Food Chem,2000,48(10):4744
[6] YAO L H,JIANG Y MD ARCY B,et al. Quantitative high performance liquid chromatography analyses of flavonoids in Australian Eucalyptus honeys[J]. J Agric Food Chem,2004, 52(2):210
[7] 王笑笑,颜伟华,黄华,等. UPLC-MS/MS法分析洋槐蜜及其蜜源花中的黄酮类成分[J].药物分析杂志, 2017, 37(5):796 WANG XX, YAN WH, HUANG H, et al, Analysis of flavonoids in acacia honey and locust flower by UPLC-MS/MS[J]. Chin J Pharm Anal, 2017, 37(5):796
[8] 王笑笑,周勇,俞婷婷,等. UPLC-MS/MS法分析10种不同蜜源蜂蜜中的黄酮类组分[J].药物分析杂志, 2016, 36(12):2180 WANG XX, ZHOU Y, YU TT, et al. Flavonoids components analysis of ten different kind of nectar honey by UPLC-MS/MS[J]. Chin J Pharm Anal,2016, 36(12):2180
[9] TRUCHADO P, FERRERES F, BORTOLOTTI L, et al. Nectar flavonol rhamnosides are floral markers of Acacia(Robinia pseudacacia)Honey[J]. J Agric Food Chem,2008,56(19):8815
[10] 刑建军,杨洁,郑国伟,等. XAD-2树脂对油菜蜂蜜中黄酮类物质分离条件的优化[J].中国蜂业, 2010, 61(12):9 XING JJ,YANG J, ZHENG GW,et al. Isolation and identification of flavonoids on rape honey through column chromatography and LC/MS/MS[J]. Apic China,2010, 61(12):9
[11] 杨洁.油菜蜂蜜中黄酮定量、定性分析及指纹图谱的初步建立[D].武汉:华中农业大学, 2011 YANG J. Identification and Quantitative Analysis of Flavonoids and the Establishment of Fingerprint in Rape Honey[D]. Wuhan:Huazhong Agricultural University,2011
[12] 齐桂平,胡月婷,杨玉杉,等.油菜蜜中5种酚类化合物的HPLCECD法测定[J].食品与发酵工业, 2010, 36(1):131 QI GP, HU YT, YANG YS, et al. Determination of five phenolic components in rape honey by HPLC-ECD[J]. Food Ferment Ind, 2010, 36(1):131
[13] ESCRICHE I, KADAR M, JUAN-BORRÁS M, et al. Using flavonoids, phenolic compounds and headspace volatile profile for botanical authentication of lemon and orange honeys[J]. Food Res Int,2011, 44(5):1504.
[14] 孙崇臻,王超,蔡子哲,等.高效液相色谱测定蜂蜜中的脱落酸、黄酮和酚酸[J].食品科学, 2013. 34(10):281 SUN CHZH,WANG CH,CAI ZZH,et al. Determinaton of flavonoids, phenolic acids and abscisic acid in honeys of different floral origins by HPLC[J]. Food Sci,2013, 34(10):281
[15] 郭夏丽,罗丽萍,冷婷婷等,7种不同蜜源蜂蜜的化学组成及抗氧化性[J].天然产物研究与开发, 2010, 22(4):665 GUO XL, LUO LP, LENG TT, et al. Chemical compositions and antioxidant activities of seven honeys from different floral sources[J]. Nat Prod Res Dev,2010, 22(4):665
[16] 杨必成,刘海,杨义芳,等.油菜花粉中黄酮类化合物的提取与分析[J].中草药,2011, 42(12):2451 YANG BC, LIU H, YANG YF, et al. Extraction and analysis of flavonoids in rape bee pollen from Brassica campestis[J]. Chin Tradit Herb Drugs,2011, 42(12):2451

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