灵芝孢子油中7个脂肪酸的含量测定

目的：建立气相色谱法测定灵芝孢子油中7个脂肪酸（肉豆蔻酸、十五烷酸、棕榈酸、十七烷酸、硬脂酸、油酸和亚油酸）含量的分析方法。方法：采用HP-FFAP（30 mm×0.32 mm×250 μm）色谱柱，程序升温的方式对灵芝孢子油中7个脂肪酸含量进行测定研究。结果：各脂肪酸线性关系良好，重复性RSD均在3.0%以下，加样回收率（n=9）在96.6%~103.3%之间；样品测定结果显示在灵芝孢子油脂肪酸中，不饱和的脂肪酸约占总量的76.41%，以油酸和亚油酸为主，饱和脂肪酸以棕榈酸和硬脂酸为主，约占总量的18.05%。结论：该方法可作为检测灵芝孢子油中7个脂肪酸的推广方法。

Objective: To develop a method for the determination of 7 fatty acids(nutmeg acid, pentadecanoic acid,palmitic acid,heptadecanoic acid,stearic acid,oleic acid,linoleic acid)in Ganoderma lucidum spore oil using gas chromatography(GC).Methods: The chromatographic column of HP-FFAP and the method of temperature programmed were adopted for quantitative analysis of 7 fatty acids in Ganoderma lucidum spore oil.Results: The linearity of 7 fatty acids in Ganoderma lucidum spore oil was good,RSD of repeatability was with less than 3.0%,and the average recoveries(n=9)were 96.6%-103.3%.The results showed that unsaturated fatty acids,rich with oleic acid and linoleic acid,accounted for about 76.41% of the total fatty acids in Ganoderma lucidum spore oil,saturated fatty acids,mainly as palmitic acid and stearic acid,accounted for about 18.05% of the total.Conclusion: The method has been successfully applied in determination of 7 fatty acids in Ganoderma lucidum spore oil.

[1] IFF B, WACHTEL-GALOR S. Herbal Medicine:Biomolecular and Clinical Aspects. 2nd Ed[M]. Boca Raton(FL):CRC Press, 2011:55
[2] 周莉云.浅析灵芝的药理和功效[J].中国民族民间医药,2013, 22(9):11 ZHOUY LY. The pharmacology and efficacy of Ganoderma lucidum[J]. Chin J Ethnomed Ethnopharm,2013, 22(9):11
[3] 张伟,曾园山.灵芝孢子的研究进展[J].中西医结合学报,2004, 2(6):463 ZHANG W,ZENG YS. The research progress of Ganoderma lucidum spores[J]. J Integr Med,2004,2(6):463
[4] 陈宝田,龙亚秋,李华,等.灵芝孢子粉的药理作用研究进展[J]. 中国药房, 2010, 21(5):1439 CHEN BT, LONG YY, LI H, et al. The research progress of pharmacological effects on Ganoderma lucidum spores[J]. China Pharm,2010, 21(5):1439
[5] 唐柳,张志军,魏雪生,等.灵芝孢子粉药理作用研究进展[J].天津农业科学, 2011, 17(3):25 TANG L, ZHANG ZJ, WEI XS, et al. Research progress of pharmacological action about spore powder of Ganoderma lucidum[J]. Tianjin Agric Sci,2011, 17(3):25
[6] YUE GGL, FUNG KP, LEUNG PC, et al. Comparative studies on the immunomodulatory and antitumor activities of the different parts of fruiting body of Ganoderma lucidum and Ganoderma spores[J]. Phytother Res,2008, 22(10):1282
[7] FUKUZAWA M,YAMAGCHI R,HIDE I,et al. Possible involvement of long chain fatty acids in the spores of Ganoderma lucidum(Reishi Houshi)to its anti-tumor activity[J]. Biol Pharm Bull, 2008, 31(10):1933
[8] OLIVEIRA M, REIS FS, SOUSA D, et al. A methanolic extract of Ganoderma lucidum fruiting body inhibits the growth of a gastric cancer cell line and affects cellular autophagy and cell cycle[J]. Food Funct,2014, 5(7):1389
[9] 卞嵩,许立,俞云,等.灵芝孢子油对H₂₂ 荷瘤小鼠肿瘤生长及瘤细胞VEGF表达的影响[J].安徽医药,2007, 11(12):1067 BIAN S, XU L, YU Y, et al. LZBZY,s regulatory effect on the growth of ₂₂ tumor and expression of VECF in transplanted H₂₂ tumor of mice[J]. Anhui Med Pham J,2007, 11(12):1067
[10] 陈娟.破壁灵芝孢子粉对裸鼠移植性淋巴瘤生长的抑制作用[J].中国实验血液学杂志, 2012, 20(2):310 CHEN J. Inhibitory effects of sporederm-broken Ganoderma lucidum spores on growth of lymphoma implanted in nude mouse[J]. J Exp Hematol,2012, 20(2):310
[11] 黄建康,王凤岩,黄琼,等.灵芝孢子油增强小鼠免疫功能及护肝作用的实验研究[J].现代预防医学,2007, 34(7):1272 HUANG JK, WANG FY, HUANG Q, et al. Experimental study on the enhancement of immune function by the oil from spores of Ganoderma lucidum and its protective effects against liver injury in mice[J]. Mod Prev Med,2007, 34(7):1272
[12] 朴日龙,崔永,许青松.灵芝孢子油对四氯化碳诱导的小鼠肝损伤的保护作[J].延边大学医学学报, 2011, 34(1):21 PIAO RL,CUI Y,XU QQ, Protective effects of the oil from spores of Ganoderma lucidum on carbon tetrachloride-induced hepatic injury in mice[J]. J Med Sci Yanbian Univ,2011, 34(1):21
[13] 李森柱,谢意珍,周静文,等.灵芝孢子油主要活性成分及降血脂功能的研究[J].中国食用菌, 2006, 25(5):40 LI SZ, XIE YZ, ZHOU JW, et al. Analysis on bioactive ingredients in Ganoderma lucidum spore oil and its effects on reducing serum lipid in vivo[J]. Edib Fungi China,2006, 25(5):40
[14] 冯敏,朱锦龙,沈建,等.灵芝孢子油诱导Jurkat细胞凋亡作用的研究[J].亚太传统医药, 2005(2):160 FENG M, ZHU JL, SHEN J, et al. Study of effects of Ganoderma lucidum spores oil induced Jurkat cells apoptosis[J]. Asia-Pacific Tradit Med,2005(2):160
[15] 高宏,徐慧,贾涛,等.气相色谱法测定氢化油脂加工食品中反式脂肪酸的含量[J].食品安全质量检测学报,2015, 6(1):321 GAO H, XU H, JIA T, et al. Determination of the trans-fatty acids in hydrogenated fat processed foods by gas chromatography[J]. J Food Saf Qual,2015, 6(1):321
[16] 黄慧敏,张伟国.反相高效液相色谱法测定苹果发酵液中的有机酸[J].食品与发酵工业, 2012, 38(5):180 HUANG HM,ZHANG GW. Reversed phase high performance liquid chromatography method for determination of organic acids in apple fermented liquid[J]. Food Ferment Ind,2012, 38(5):180
[17] 林晓婕,魏巍,何志刚,等.离子排斥色谱法测定黄酒中的13种有机酸[J].色谱,2014, 32(3):304 LIN XJ,WEI W,HE ZG,et al. Determination of organic acid in rice wine by ion-exclusion chromatography[J]. Chin J Chromatogr, 2014, 32(3):304
[18] 李永库,刘衣南,吕琳琳,等.毛细管电泳-质谱联用法测定葡萄酒中8种有机酸含量[J].质谱学报, 2013, 34(5):288 LI YK, LIU YN, LU LL, et al. Simultaneous determination of eight organic acid in grape wines by capillary electrophoresis-electrospray ionization mass spectrometry[J]. J Chin Mass Spectrom Soc,2013, 34(5):288