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刊物信息

期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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色谱柱表征体系在选择最佳反相色谱柱中的应用

Application of column characterization systems in selecting optimal RP-HPLC columns

作者: 张夏, 胡昌勤 
作者(英文):
分类号:R917
出版年·卷·期(页码):2017,37 (6):942-949
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

反相高效液相色谱法是目前药物分析中最常用的分析技术。不同色谱柱选择性的差异,常导致溶质的保留值、色谱峰分离度甚至色谱峰顺序出现较大差异。因此,寻找到性能适宜的色谱柱关系到整个色谱实验的成败。利用色谱柱参数寻找选择性相似或者互补色谱柱指导实际分离工作,推动了色谱柱选择模式的发展。本文综述了近年来基于疏水消除模型的色谱柱表征体系在色谱柱选择中应用的进展,主要包括选择相似或互补的色谱柱,选择最佳分离色谱柱,选择UHPLC色谱柱,并对目前各选择方式的局限性进行了讨论。

-----英文摘要:---------------------------------------------------------------------------------------

Reversed-phase high performance liquid chromatography has been the most commonly used method in pharmaceutical analysis so far.The diversities of column selectivity often lead to the changes of solute retention time,resolution and peak order.Therefore,it is the suitable chromatographic column that affects the success of the experiment.Using column parameters to search for equivalent or orthogonal columns promotes the development of rational selection of columns.This article summarizes the recent progress on applications of column characterization systems in the rational selection of RP-HPLC columns based on hydrophobic subtraction model,including the selection of equivalent or orthogonal columns,optimal chromatographic columns and UHPLC columns.Meanwhile, the existing problems about aforementioned applications are also discussed in this article.

-----参考文献:---------------------------------------------------------------------------------------

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