HPLC法同时测定厚朴排气合剂中和厚朴酚、厚朴酚、大黄素和大黄酚含量

目的：建立HPLC法同时测定厚朴排气合剂中和厚朴酚、厚朴酚、大黄素和大黄酚。方法：采用Syncronis C₁₈（250 mm×4.6 mm，5 μm）色谱柱，以甲醇（A）-0.1%磷酸水（B）为流动相，梯度洗脱（0~15 min，80% A→85% A；15~40 min，85% A→95% A），流速1.0 mL·min^-1，检测波长254 nm，柱温30℃，进样量20 μL。结果：和厚朴酚、厚朴酚、大黄素和大黄酚的保留时间分别为13.1、19.0、24.1和32.1 min，线性范围分别为0.73~8.76、1.06~12.69、0.09~1.04、0.29~3.48 mg·mL^-1；平均回收率分别为100.1%、105.2%、103.2%和97.5%，RSD分别为5.7%、3.4%、6.0%和2.3%。5批厚朴排气合剂中和厚朴酚、厚朴酚、大黄素和大黄酚含量分别为17.26~18.05、25.34~26.11、2.01~2.10和1.72~1.81 mg·mL^-1。结论：本法可为厚朴排气合剂的质量控制提供参考。

Objective: To study an HPLC method for simultaneous determination of honokiol,magnolol,and chrysophanol in Houpopaiqi mixture by HPLC. Methods: The chromatographic separation was performed on a Syncronis C₁₈(250 mm×4.6 mm,5 μm)column with the mobile phase of methanol(A)-0.1% aqueous phosphoric acid(B)by gradient elution(0-15 min,80%A→85%A;15-40 min,85%A→95%A).The flow rate was 1.0 mL·min^-1.The detection wavelength was 254 nm.The column temperature was 30℃,and the injection volume was 20 μL. Results: The retention time of honokiol,magnolol,emodin and chrysophanol were 13.1,19.0,24.1 and 32.1 min,respectively;the linear ranges were 0.73-8.76 mg·mL^-1,1.06-12.69 mg·mL^-1,0.09-1.04 mg·mL^-1 and 0.29-3.48 mg·mL^-1,respectively;the recoveries were 100.1%,105.2%,103.2% and 97.5% and the RSDs were 5.7%, 3.4%,6.0% and 2.3%,respectively.The contents of honokiol,magnolol,emodin and chrysophanol in 5 Houpopaiqi mixture were 17.26-18.05 mg·mL^-1,25.34-26.11 mg·mL^-1,2.01-2.10 mg·mL^-1 and 1.72-1.81 mg·mL^-1,respectively. Conclusion: The method can be used as a reference for the quality control of Houpopaiqi mixture.

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