可调激光-微顺序注射-阀上实验室荧光反猝灭法测定硫酸氢氯吡格雷的含量

目的：采用可调激光-微顺序注射-阀上实验室荧光反猝灭法测定硫酸氢氯吡格雷含量。方法：四苯硼钠与罗丹明B反应，荧光信号猝灭，加硫酸氢氯吡格雷后，其与四苯硼钠-罗丹明B体系中的四苯硼钠反应形成更稳定的疏水性离子缔合物，使罗丹明B重新释放出荧光，检测荧光信号的增强程度（ΔF），通过ΔF与硫酸氢氯吡格雷的浓度（C）的回归方程，计算硫酸氢氯吡格雷含量。结果：荧光信号的ΔF与硫酸氢氯吡格雷C线性回归方程为ΔF=4 471.7C+12 552，r=0.998 4，线性范围为0.7~28.2 μmol·L^-1，检出下限为0.067 μmol·L^-1，重复性RSD为2.9%，加样回收率在93.0%~99.5%之间。用该方法对市售硫酸氢氯吡格雷片进行测定，结果为97%~100%。结论：该方法可用于测定药物中硫酸氢氯吡格雷含量。

Objective: To analyze clopidogrel hydrogen sulfate by fluorescence quenching method in the laboratory of a tunable laser micro sequential injection valve(MicroSIA Lab-on-valve,μSIA-LOV)method. Methods: Rhodamine B had fluorescence quenching effect at stable conformation with sodium tetraphenylboron.When clopidogrel hydrogen sulfate reacted with sodium tetraphenylboron to form a more stable hydrophobic ion association complex,rhodamine B was released and fluorescence intensity of solution was enhanced(ΔF).The fluorescence signal enhancement ΔF had good linearity with the concentration(C)of clopidogrel hydrogen sulfate. Results: the linearity equation of this method was ΔF=4 471.7C+12 552,r=0.998 4,the range was 0.7-28.2 μmol·L^-1,detection limit was 0.067 μmol·L^-1,the RSD of this method was 2.9%,and the standard added recovery rates were between 93.0%-99.5%.This method was applied to the quantitative analysis of commercially clopidogrel bisulfate tablets.The results were 97%-100%. Conclusion: This method can be used for quality control of clopidogrel hydrogen sulfate.

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