HPLC法同时测定不同产地掌叶大黄中10个蒽醌类化合物

目的：建立高效液相色谱法同时测定不同产地掌叶大黄中10个蒽醌类成分（芦荟大黄素-8-O-葡萄糖苷、大黄酸-8-O-β-D-葡萄糖苷、大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-O-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷和芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚）。方法：采用C₁₈色谱柱（250 mm×4.6 mm，5 μm），甲醇-0.1%磷酸水为流动相，梯度洗脱，流速为1.0 mL·min^-1，检测波长254 nm，柱温25℃。结果：10个成分在测定范围内线性关系较好（r>0.999），芦荟大黄素-8-O-葡萄糖苷、大黄酸-8-O-β-D-葡萄糖苷、大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-O-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷和芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚平均回收率（n=6）分别为99.0%、99.2%、99.0%、99.2%、99.0%、99.2%、98.8%、99.4%、98.7%、99.6%；不同产地大黄药材中上述10个蒽醌类成分含量差异明显，含量范围分别为0.044%~0.130%、0.348%~1.686%、0.028%~0.183%、0.164%~0.352%、0~0.116%、0.095%~0.161%、0.294%~0.411%、0.157%~0.265%、0.275%~0.321%、0.064%~0.150%。结论：本文建立的方法样品处理简便，分析灵敏，能为大黄药材的质量评价提供参考。

Objective: To establish simultaneous determination method of ten anthraquinones(aloe-emodin-8-O-glucopyranoside,rhein-8-O-glucopyranoside,chryohol-8-O-β-D-glucoyroide,emodin-8-O-glucoside, physcion-8-O-β-D-monoglucoside,aloe-emodin,rhein,emodin,chrysophanol and physcion)in Rheum palmatum L. from different habitants by HPLC. Methods: C₁₈ column(250 mm×4.6 mm,5 μm)was adopted,the mobile phase was a mixture of methanol and 0.1% phosphoric acid at the flow rate of 1.0 mL·min^-1,the determination wavelength was set at 254 nm,the column temperature was 25℃ and the injection volume was 10 μL. Results: In the determination range,the linear relationship of ten components was good,and the recoveries of aloe-emodin-8-O-glucopyranoside,rhein-8-O-glucopyranoside,chryohol-8-O-β-D-glucoyroide,emodin-8-O-glucoside,physcion-8-O-β-D-monoglucoside,aloe-emodin,rhein,emodin,chrysophanol and physcion were 99.0%,99.2%,99.0%,99.2%,99.0%,99.2%,98.8%,99.4%,98.7% and 99.6%,respectively.The contents of ten anthraquinones in Rhei Radix et Rhizoma from different habitants were 0.044%-0.130%,0.348%-1.686%,0.028%-0.183%,0.164%-0.352%,0-0.116%,0.095%-0.161%,0.294%-0.411%,0.157%-0.265%,0.275%-0.321% and 0.064%-0.150%,which showed remarkable difference. Conclusion: The proposed method was simple and sensitive,thus providing reference for the quality evaluation of Rheum palmatum L.

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