Objective:To establish methods for distribution and encapsulation efficiency of dexamethasone in eye drops with lipid emulsion as carrier in different phases,and to analyze the feasibility of this method used in drug performance evaluation. Methods:Based on the differences of density in each phase of lipid emulsion,centrifuge with 8℃,30 000 r·min-1 was used to separate the drug in oil,in water and in oil/water interface phase. The free drug on the surface and drugs coated in the emulsion droplet were separated and determined by micro column centrifugation using gel molecular sieve method. A Sun Fire C18 column (250 mm×4. 6 mm, 5 μm) was adopted, the mobile phase was acetonitrile-water(28:72) at the flow rate of 1. 0 mL·min-1, the detection wavelength was 240 nm, the column temperature was 30℃,and the sample volume was 20 μL. Results:The distribution of dexamethasone in water phase, oil phase and lecithin layer at oil/water interface were 42.43%, 31. 12% and 24.45%, respectively. The encapsulation efficiency of dexamethasone in emulsion droplet was 50. 11%. Conclusion:The phase distribution and entrapment efficiency can accurately evaluate the performance of drugloading in eye drops with lipid emulsion as carrier,and the established method can be used for quality control.
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