UPLC同时测定鸡骨草中2种生物碱和2种黄酮碳苷的含量

目的：建立UPLC法同时测定鸡骨草中相思子碱、下箴刺桐碱、新西兰牡荆苷2、夏佛塔苷含量，为药材质量标准改进提供方法。方法：采用HSS T3色谱柱（100 mm×2.1 mm，1.8 μm），以乙腈（A）-0.2%甲酸溶液（B）为流动相进行梯度洗脱，流速0.3 mL·min^-1，检测波长270 nm，柱温30℃。结果：相思子碱、下箴刺桐碱、新西兰牡荆苷2、夏佛塔苷的质量浓度分别在10.20~102.00 μg·mL^-1（r=0.999 2）、6.88~68.80μg·mL^-1（r=0.999 0）、5.40~54.00 μg·mL^-1（r=0.999 9）、10.92~109.20 μg·mL^-1（r=0.999 3）的范围内线性关系良好；各成分平均加样回收率为99.1%（RSD=1.3%）、101.1%（RSD=0.69%）、103.8%（RSD=0.81%）、100.9%（RSD=0.75%）。5批样品中相思子碱、下箴刺桐碱、新西兰牡荆苷2、夏佛塔苷含量范围分别为23.02~67.24、14.98~40.09、3.02~22.51、6.34~111.38 μg·g^-1。结论：该法经方法学验证，可同时测定鸡骨草中4种成分的含量，可为该药材的质量标准改进提供参考。

Objective: To establish a UPLC method for simultaneous determination of abrine, hypaphorine, vicenin-2 and schaftoside in Abri Herba,and to improve the quality standard of the drug. Method:Separation was performed on a HSS T3 column(100 mm×2.1 mm,1.8μm) and the mobile phase was acetonitrile-0.2% formic acid with gradient elution. The flow rate was 0.3 mL·min^-1 and the wavelength was 270 nm. The column temperature was 30℃. Results: The linear ranges of abrine,hypaphorine,vicenin-2,schaftoside were 10.20-102.00 μg·mL^-1(r=0.999 2), 6. 88-68.80 μg·mL^-1(r=0.999 0), 5.40-54.00 μg·mL^-1(r=0.999 9) and 10.92-109.20μg·mL^-1(r=0.999 3), respectively. The average recoveries(n=6) were 99.1%(RSD=1.3%), 101.1%(RSD=0.69%), 103.8%(RSD=0.81%) and 100.9%(RSD=0.75%), respectively. The content ranges of abrine,hypaphorine,vicenin-2 and schaftoside in Abri Herba were 23.02-67.24 μg·g^-1,14.98-40.09 μg·g^-1, 3.02-22.51 μg·g^-1 and 6.34-111.38 μg·g^-1, respectively. Conclusion: Method validation suggested that the developed method was suitable for simultaneous determination of 4 components in Abri Herba,thus providing reference for the improvement of quality standard of the drug.

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