Objective: To establish a multi-wavelength UPLC method for simultaneous determination of four index components(gallic acid, spent acid, quercetin and kaempferol) in Euphorbiae Humifusae Herba, and to compare the difference among samples from different habitants. Methods: The analysis was performed on a 30℃ thermostatic Acquity UPLC BEH C18 column(50 mm×2.1 mm, 1.7 μm), with the mobile phase consisting of acetonitrile and 0.2% phosphoric acid by gradient elution. The flow rate was 0.2 mL·min-1 and the detection wavelengths were 271 nm(0-2 min), 254 nm(2-6 min) and 360 nm(6-8 min). Results: Baseling resolved peaks were achieved for four components in 8 minutes. The linear ranges of gallic acid, spent acid, quercetin and kaempferol fell within the ranges of 65.88-164.70 μg·mL-1(R2=0.999 2), 149.10-372.75 μg·mL-1 (R2=0.999 2), 27.48-68.70 μg·mL-1(R2=0.999 7) and 30.24-75.60 μg·mL-1(R2=0.999 2), respectively. The average recoveries(n=6) were 99.5%(RSD=1.4%), 98.3%(RSD=0.92%), 99.1%(RSD=1.2%) and 99.3%(RSD=1.4%), respectively. The contents of four components in 6 batches for samples were 4.148-7.663 mg·mL-1, 9.727-17.388 mg·mL-1, 1.763-2.977 mg·mL-1 and 0.684-2.037 mg·g-1, respectively. Conclusion: This paper revealed difference in contents of indicative components in Euphorbiae Humifusae Herba, thus providing a theoretical basis for the quality control of the herb.
[1] 中国药典2010年版.一部[S]. 2010:50 ChP 2010. Vol Ⅰ[S]. 2010:50
[2] 王婷婷,文今福,金松南.地锦草的化学成分及药理作用研究进展[J].泰山医学院学报, 2012, 33(8):629 WANG TT,WEN JF,JIN SN. Studies on chemical constituents and pharmacological activities of Euphorbia humifusa[J]. J Taishan Med Coll,2012, 33(8):629
[3] 谢奇,李治建,斯拉甫·艾白,等.地锦草化学成分及其抗真菌作用的研究进展[J].医药导报, 2011, 30(7):880 XIE Q, LI ZJ, AIBAI SLP, et al. Research progress on chemical constituents of Euphorbia humifusa and its antifungal activity[J]. Her Med,2011, 30(7):880
[4] 褚小兰,范崔生.地锦类中草药的化学成分和药理研究概况[J].中国野生植物资源,1998, 17(2):17 CHU XL, FAN CS. Overview of research on chemical constituents and pharmacological in Euphorbia humifusa herb[J]. Chin Wild Plant Resour, 1998, 17(2):17
[5] 柳润辉,孔令义.地锦草脂溶性成分研究[J].天然产物研究与开发, 2005, 17(4):437 LIU RH, KONG LY. Lipid constituents from Euphorbia humifusa Willd.[J]. Nat Prod Res Dev,2005, 17(4):437
[6] 田瑛,孙立敏,刘细桥,等.中药地锦草酚性成分[J].中国中药杂志, 2010, 35(5):613 TIAN Y, SUN LM, LIU XQ, et al. Phenolic constituents of Euphorbia humifusa Willd.[J]. China J Chin Mater Med, 2010, 35(5):613
[7] 朱英.地锦草的研究进展[J].现代中药研究与实践,2003, 17(5):62 ZHU Y. Research progress of Euphorbia humifusa[J]. Res Pract Chin Med,2003, 17(5):62
[8] YOSHIDA T, AMAKURA Y, LIU YZ, et al. Tannins and related polyphenols of euphorbiaceous plants. Ⅺ. Three new hydrolyzable tannins and a polyphenol glucoside from Euphorbia humifusa[J]. Chem Pharm Bull,1994, 42(9):1803
[9] LI ZJ,GUO X, DAWUTI G,et al. Antifungal activity of ellagic acid in vitro and in vivo[J]. Phytother Res,2015, 29(7):1019
[10] 李沐涵,殷美琦,冯靖涵,等.没食子酸抗肿瘤作用研究进展[J].中医药信息, 2011, 28(1):109 LI MH, YIN MQ, FENG JH, et al. Research progress on antitumor effect of gallic acid[J]. Inf Tradit Chin Med,2011, 28(1):109
[11] 丁运生,孙小虎,李有桂,等.鞣花酸及其衍生物研究进展[J]. 合肥工业大学学报(自然科学版),2008, 31(11):1809 DING YS, SUN XH,LI YG,et al. Research progress of ellagic acid and its derivatives[J]. J Hefei Univ Technol (Nat Sci), 2008, 31(11):1809
[12] 张志琴,朱双雪.槲皮素的药理活性与临床应用研究进展[J]. 药学研究,2013, 32(7):400 ZHANG ZQ, ZHU SX. Research progress on pharmacological activities and clinical application of quercetin[J]. J Pharm Res, 2013, 32(7):400
[13] 陈育华,周克元,袁汉尧.山柰酚药效的研究进展[J].广东医学,2010, 31(8):1064 CHEN YH, ZHOU KY, YUAN HR. Research progress of the effect of kaempferol[J]. Guangdong Med J,2010, 31(8):1064
[14] 冯光维,刘志东,祁东利,等. UPLC法同时测定地锦草中4种指标成分的含量[J].天津中医药大学学报, 2016, 35(3):196 FENG GW, LIU ZD, QI DL, et al. Simultaneous determination of 4 components in Herba Euphorbiae by UPLC[J]. J Tianjin Univ Tradit Chin Med,2016, 35(3):196
[15] 吕凤莲,宋韶锦,方彬. HPLC法测定地锦草中芦丁的含量[J]. 齐鲁药事, 2010, 29(3):139 LÜ FL, SONG SJ, FAGN B. Determination of rutin in Herba Euphorbiae by HPLC[J]. Qilu Pharm Aff,2010, 29(3):139
[16] 贾乐陶,陈进,张宏业.地锦草胶囊中槲皮素的含量测定[J].中国药业, 2007, 16(13):28 JIA LT,CHEN J,ZHANG HY. Determination of quercetin in Herba Euphorbiae capsule[J]. China Pharm,2007, 16(13):28
[17] 黄彩虹,刘海涛,雷鹏,等.高效液相色谱法测定地锦草中鞣花酸的含量[J].中南药学, 2013, 11(2):136 HUANG CH,LIU HT,LEI P,et al. Determination of ellagic acid in Herba Euphorbiae by HPLC[J]. Cent South Pharm,2013, 11(2):136
[18] 林辉. HPLC测定地锦草中没食子酸和芦丁的含量[J].中成药, 2009, 31(9):1465 LIN H. HPLC determination of the gallic acid and rutin in Euphorbiae Humifusae Herba[J]. Chin Tradit Pat Med,2009,31(9):1465
