Objective: To establish a method for studying the structure of polysorbate 80.Methods: Nuclear magnetic resonance(NMR)spectra were collected at Bruker Ascend 500 spectrometer with 5 mm BBO probe at 25℃. In 1D 1H-NMR spectra, the relaxation delay(D1) was 2 s, spectral width(SW) was δH 15, data point was 64 k and number of scan was 128. In 1D 13C-NMR spectra, the D1 was 1 s, SW is was δC 236.6, data point was 64 k and number of scan was 32 768. In 2D NMR spectra, the D1 was 1.4 s and the number of scan was 16.Results: The structure of polysorbate 80 was characterized by 1H-NMR, 13C-NMR, HSQC-NMR, HMBC-NMR, COSYNMR spectraConclusion: NMR spectra are specific, simple and convenient method for studying the structure of polysorbate 80.
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