Objective: To establish an HPLC method for simultaneous determination of the content of five saponins(bacopaside I, bacoside A3, Bacopaside Ⅱ, bacopasaponin C isomer and bacopasaponin C)in Bacopa monnieri.Methods: Bacopa monnieri was extracted with 50% ethanol. Separation was performed on an Agela Promsil C18 column(4.6 mm×250 mm, 5 μm). And the mobile phase Mobile phase consisted of acetomitrile-0.2% phosphoric acid(pH was adjusted to 3.0 by sodium hydroxide solution)(32.5:67.5)at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 205 nm and the column temperature was maintained at 30℃.Results: Five saponins showed good linear relation(r≥0.999 6)and their average recoveries were between 98.6% and 100.4%. Contents of five saponins in Bacopa monnieri from different habitats were different from each others. The total content of five components in 10 samples were in the range of 4.22-5.62 mg·g-1, 1.61-3.04 mg·g-1, 2.62-4.94 mg·g-1, 0.38-4.53 mg·g-1 and 2.14-6.33 mg·g-1, respectively.Conclusion: This method could be applied to the quality control of B. monnieri.
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