HPLC法测定甜叶菊中6个酚酸类成分含量

目的：建立高效液相色谱法同时测定甜叶菊叶中新绿原酸、绿原酸、隐绿原酸、异绿原酸B、异绿原酸A和异绿原酸C6个酚酸类成分。方法：采用C₁₈色谱柱（4.6 mm×250 mm，5μm），流动相为0.1%甲酸水溶液-乙腈，梯度洗脱，柱温30℃，检测波长327 nm。结果：6个酚酸质量浓度在10~196 μg·mL^-1范围内线性关系良好；6个酚酸的加样回收率分别为98.3%、95.2%、100.1%、97.3%、95.6%和96.1%，加样回收率、精密度、重复性、稳定性均符合有关规定；9批样品中6个酚酸类成分的含量分别为新绿原酸0.062%~0.725%，绿原酸0.277%~2.450%，隐绿原酸0.096%~0.694%，异绿原酸B 0.046%~0.867%，异绿原酸A0.157%~0.772%，异绿原酸C 0.135%~1.821%，6个酚酸之和在1.182%~6.668%。结论：该方法可为甜叶菊进一步开发利用提供质量控制方法。

Objective: To establish an HPLC method for simultaneous determination of six phenolic acids including 5-caffeoylquinic acid, chlorogenic acid, 4-caffeoylquinic acid, 3, 4-dicaffeoylquinic acid, 3, 5-dicaffeoylquinic acid and 4, 5-dicaffeoylquinic acid in the leaves of Stevia rebaudiana Bertoni.Methods: The analyses were performed on a C₁₈ column(4.6 mm×250 mm, 5μm)with the gradient elution of acetonitrile-0.1% formic acid water at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 327 nm and the column temperature was set at 30℃.Results: The six phenolic acids have good linearity in the concentration range of 10-196 μg·mL^-1. The average recoveries of the six compounds were 98.3%, 95.2%, 100.1%, 97.3%, 95.6% and 96.1%, respectively. The contents of six phenolic acids in 9 samples were 0.062%-0.725%(5-caffeoylquinic acid), 0.277%-2.450%(chlorogenic acid), 0.096%-0.694%(4-caffeoylquinic acid), 0.046%-0.867%(3, 4-dicaffeoylquinic acid), 0.157%-0.772% (3, 5-dicaffeoylquinic acid)and 0.135%-1.821%(4, 5-dicaffeoylquinic acid), respectively. The sum of the six phenolic acids is 1.182%-6.668%.Conclusion: The HPLC method is helpful for the quality control of the leaves of Stevia rebaudiana Bertoni.

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