HPLC法同时测定老蔻丸中8个成分的含量

目的：建立HPLC法同时测定老蔻丸（陈皮、肉桂、丁香等）中8个成分的含量。方法：药物的甲醇提取液的采用依利特SinoChrom ODS-BP C₁₈色谱柱（200 mm×4.6 mm，5 μm）；流动相为乙腈-水-甲醇，梯度洗脱；流速为1.0 mL·min^-1，柱温为30℃，检测波长为280 nm，进样量为15 μL。结果：柚皮苷、橙皮苷、新橙皮苷、桂皮醛、丁香酚、和厚朴酚、厚朴酚、大黄酚质量浓度分别在26.669~400.031、26.803~402.042、13.335~200.028、2.566~38.484、4.272~64.086、19.994~299.908、26.682~400.223、1.068~16.014范围内与各自峰面积积分值呈良好的线性关系，相关系数r值均大于0.999；平均加样回收率（n=6）分别为100.7%、99.9%、100.1%、100.9%、99.9%、99.9%、100.6%、100.4%，RSD分别为0.77%、0.65%、0.55%、1.3%、1.4%、0.70%、0.56%、1.3%，3批样品中柚皮苷、橙皮苷、新橙皮苷、桂皮醛、丁香酚、和厚朴酚、厚朴酚、大黄酚的含量分别为1 487.42~1 678.33、6 203.50~6 982.31、1 272.29~1 410.19、236.49~292.03、1 869.33~1 896.70、3 767.62~4 073.41、2 879.84~3 250.23、279.55~301.75 μg·g^-1。结论：该方法灵敏度高，专属性好，可用于老蔻丸的质量控制。

Objective: To establish an HPLC method for the simultaneous determination of eight constituents in Laokou pills(Citri Reticulatae Pericarpium,Cinnamomi Cortex and Caryophylli Flos,etc.) Methods: The analysis of methanol extract of this drug was carried out on an Elite Sino Chrom ODS-BP C₁₈(200 mm×4.6 mm,5 μm) column at 30℃,with the mobile phase comprising of acetonitrile-water-methanol at a flow rate of 1.0 mL·min^-1 in a gradient elution. And the detection wavelength was set at 280 nm. The injection volume was 15 μL. Results: Naringin,hesperidin,neohesperidin,cinnamaldehyde,eugenol,honokiol,magnolol and chrysophanol showed good linear relationships within the ranges of 26.669-400.031,26.803-402.042,13.335-200.028,2.566-38.484,4.272-64.086,19.994-299.908,26.682-400.223 and 1.068-16.014 μg·mL^-1,respectively with correlation coefficients above 0.999. And the average recoveries(n=6) were 100.7%,99.9%,100.1%,100.9%,99.9%,99.9%,100.6% and 100.4%,with the RSDs of 0.77%,0.65%,0.55%,1.3%,1.4%,0.70%,0.56% and 1.3%,respectively. The contents of naringin,hesperidin,neohesperidin,cinnamaldehyde,eugenol,honokiol,magnolol and chrysophanol in three batches of samples were 1 487.42-1 678.33,6 203.50-6 982.31,1 272.29-1 410.19,236.49-292.03,1 869.33-1 896.70,3 767.62-4 073.41,2 879.84-3 250.23 and 279.55-301.75 μg·g^-1,respectively. Conclusion: The method has high sensitivity and good specificity,and can be used for the quality control of Laokou pills.