高效液相色谱法同时测定蒙药复方述达格-4中6个成分的含量

目的：建立采用HPLC同时测定蒙药复方述达格-4中1-苯基-7-（3-甲氧基-4-羟基）苯基-5-醇-3-庚酮、高良姜素、山柰素、高良姜素-3-甲醚、α-细辛醚、木香烃内酯含量的方法。方法：采用C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈-0.2%乙酸水溶液为流动相，梯度洗脱，流速0.6 mL·min^-1，检测波长254 nm，柱温30℃，进样量为10 μL。结果：1-苯基-7-（3-甲氧基-4-羟基）苯基-5-醇-3-庚酮、高良姜素、山柰素、高良姜素-3-甲醚、α-细辛醚、木香烃内酯的进样量与其峰面积具有良好的线性关系（r=0.999 9）；平均加样回收率（n=6）为96.6%~101.1%，RSD为0.70%~1.8%；测定样品6批，1-苯基-7-（3-甲氧基-4-羟基）苯基-5-醇-3-庚酮、高良姜素、山柰素、高良姜素-3-甲醚、α-细辛醚、木香烃内酯的平均含量分别为0.008 4%~0.060 0%、0.010 7%~0.061 6%、0.010 9%~0.086 5%、0.006 2%~0.066 6%、0.011 0%~0.078 1%、0.005 1%~0.060 6%。结论：该方法可用于蒙药复方述达格-4的质量控制，可为蒙药复方述达格-4资源开发利用提供理论依据。

Objective:To establish a method for simultaneous determination of 6 components including 1-phenyl-7-(3-methoxy-4-hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, alpha-asarone and costunolide in Mongolian medicine compound formula Shudage-4 by HPLC. Methods:Separation was performed on a C₁₈ column(4.6 mm×250 mm, 5 μm) and the mobile phase consisted of acetonitrile-0.2% glacial acetic acid with gradient elution at a flow rate of 0.6 mL·min^-1. The detection wavelength was 254 nm and the column temperature was 30℃. The injection volume was 10 μL. Results:1-phenyl-7-(3-methoxy-4-hydroxy) phenyl-5-ol-3-heptanone, galangin, kaempferide, galangin-3-methylether, alpha-asarone and costunolide exhibited good linear relationship with correlation coefficients of 0.999 9, the recoveries were 96.6%-101.1%, and the RSD were 0.70%-1.8%. The content ranges of the above mentioned components in 6 samples were 0.008 4%-0.060 0%, 0.010 7%-0.061 6%, 0.010 9%-0.086 5%, 0.006 2%-0.066 6%, 0.011 0%-0.078 1% and 0.005 1%-0.060 6%, respectively. Conclusion:The method can be applied in quality control of Mongolian medicine compound formula Shudage-4. The study can provide theoretical basis for development and utilization of Mongolian medicine Shudage-4.