HPLC法同时测定珠子草中没食子酸、短叶苏木酚、柯里拉京、鞣花酸和芦丁的含量

目的：建立反相高效液相色谱法同时测定珠子草中没食子酸、短叶苏木酚、柯里拉京、鞣花酸和芦丁5个化学成分的含量，并对方法学进行考察。方法：采用菲罗门Gemini C₁₈柱（250 mm×4.6 mm，5 μm）；流动相：乙腈（A）-0.1%磷酸水溶液（B），梯度洗脱（0~20 min，3% A→15% A；20~45 min，15% A→28% A；45~55 min，100% A；56~65 min，3% A）；流速：1 mL·min^-1；柱温：室温；检测波长：254 nm。结果：没食子酸、短叶苏木酚、柯里拉京、鞣花酸和芦丁的进样量分别在0.072~1.84 μg、0.172~4.32 μg、0.124~3.12 μg、0.076~1.92 μg、0.156~3.97 μg范围内与峰面积呈良好的线性关系。测定珠子草药材粉末5份，没食子酸、短叶苏木酚、柯里拉京、鞣花酸、芦丁平均含量分别为0.21%、0.53%、0.24%、0.41%、0.11%。结论：本方法经方法学验证可用于珠子草多成分质量评价方法。

Objective: To establish and validate the HPLC method for simultaneous determination of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin in Phyllanthus niruri.Linn.Method: Gemini C₁₈ (250 mm×4.6 mm, 5μm)column was adopted with mobile phase consisting of acetonitrile (A)and 0.1% phosphoric acid (B)in gradient elution (0-20 min, 3%A→15%A;20-45 min, 15%A→28%A;45-55 min, 100%A;56-65 min, 3%A).The flow rate was 1 mL·min^-1, the column was kept at room temperature, and the detection wavelength was 254 nm.Result: The linear ranger of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin were 0.072-1.84 μg, 0.172-4.32 μg, 0.124-3.12 μg, 0.076-1.92 μg and 0.156-3.97 μg, respectively.The average contents of galic acid, brervifolincaboxylic acid, corilagin, ellagic acid and rutin in 5 samples were 0.21%, 0.53%, 0.24%, 0.41% and 0.11%.Conclusion: This method was proved to be suitable for multi-component determination of Phyllanthus niruri.Linn.