柱前衍生化-反相高效液相色谱法测定多层共挤输液膜中抗氧剂三（壬苯基）亚磷酸盐的迁出量

目的：建立柱前衍生化-反相高效液相色谱法，对水、0.9%氯化钠注射液、pH 3.5缓冲液、pH 8.0缓冲液和15%乙醇这5种提取介质中抗氧剂三（壬苯基）亚磷酸盐（TNPP）的含量进行测定。方法：通过叔丁基过氧化氢进行柱前衍生化，采用C₈（4.6 mm×150 mm，5 μm）色谱柱，以乙腈-异丙醇（90∶10）为流动相，流速1 mL·min^-1，检测波长220 nm。结果：抗氧剂TNPP的检测下限为0.4 mg·L^-1，质量浓度在0.9~18 mg·L^-1范围内线性关系良好，5种提取介质中回收率介于92.0%~100.5%，RSD均小于1.7%，溶液24 h内稳定性良好。结论：采用柱前衍生化，不仅提高了溶液的稳定性，而且缩短了分析时间，提高了分析方法灵敏度。

Objective:To establish a RP-HPLC method with pre-column derivation for the determination of tris(nonylphenyl)phosphite(TNPP)in different extraction solvents,such as water,0.9% sodium chloride injection,pH 3.5 buffer,pH 8.0 buffer,15%(v/v)ethanol aqueous solution. Methods:TNPP was derivatized with tert-butyl hydroperoxide and analyzed by RP-HPLC,which was performed on a column of C₈ column(4.6 mm×150 mm,5 μm)with the mobile phase of acetonitrile - isopropyl alcohol (90:10)at a flow rate of 1 mL·min^-1,and at a detection wavelength of 220 nm. Results:The limit of detection was 0.4 mg·L^-1. The linear range was from 0.9 mg·L^-1 to 18 mg·L^-1.The recovery was between 92.0% and 100.5% with coefficient of variation lower than 1.7% in this five extraction solvent.The test solution was stable within 24 h.Conclusion:By pre-column derivation,not only the stability problem was solved,but also the analysis time and sensitivity were improved.