高效液相色谱-电雾式检测器法测定银杏叶提取物中萜类内酯含量

目的：建立采用高效液相色谱-电雾式检测器（HPLC-CAD）测定银杏叶提取物中萜类内酯含量的方法。方法：采用CAPCELL PAK MG Ⅱ C₁₈色谱柱（4.6 mm×250 mm，5 μm），柱温40℃，以甲醇-水（30∶70）为流动相，流速1.0 mL·min^-1；以CAD检测器检测，雾化温度40℃。结果：本方法对白果内酯、银杏内酯C、银杏内酯A、银杏内酯B的定量下限分别为14、14、28和27 ng，检测下限分别为4、6、10和10 ng；各萜类内酯线性关系良好，相关系数在0.999 6~0.999 8之间；加样回收率（n=6）分别为101.6%、95.4%、102.1%和105.1%，重复性试验的RSD分别为2.6%、3.0%、2.6%和3.0%。3批次银杏叶提取物中白果内酯、银杏内酯C、银杏内酯A和银杏内酯B含量分别为3.04%~3.18%、1.05%、1.22%~1.24%和0.63%~0.64%。结论：该方法可用于银杏叶提取物中萜类内酯的含量测定，且该方法具有更高的灵敏度。

Objective:To establish a high performance liquid chromatography(HPLC)-charged aerosol detector(CAD)method for the determination of the terpene lactones in ginkgo leaf extract.Methods:HPLC analysis was performed on a CAPCELL PAK C₁₈ MG Ⅱ column(4.6 mm×250 mm,5 μm)with the mobile phase of methanol-water(30:70)at a flow rate of 1.0 mL·min^-1,and the column temperature was 40℃.CAD was equipped as the detector with the evaporation temperature of 40℃.Results:The limit of quantification for bilobalide,ginkgolide C,ginkgolide A and ginkgolide B were 14 ng,14 ng,28 ng and 27 ng,respectively and the detection limits were 4 ng,6 ng,10 ng and 10 ng,respectively.There was a good linear relationship for the four terpene lactones.The average recoveries for bilobalide,ginkgolide C,ginkgolide A and ginkgolide B were 101.6%,95.4%,102.1% and 105.1%,and RSD values of repeatability test were 2.6%,3.0%,2.6% and 3.0%.The contents of bilobalide,ginkgolide C,ginkgolide A and ginkgolide B in 3 samples were 3.04%-3.18%,1.05%,1.22%-1.24% and 0.63%-0.64%,respectively. Conclusion:The established method can be applied to the determination of terpene lactones in ginkgo leaves extract with higher sensitivity.

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