目的：建立洋常春藤及其提取物中常春藤萜苷C、α-常春藤素、常春藤萜苷D、常春藤皂苷B等4个主要皂苷成分一测多评定量检测法。方法：以常春藤萜苷C为参照成分，建立该成分与α-常春藤素、常春藤萜苷D、常春藤皂苷B的相对校正因子，采用校正因子计算这3个常春藤皂苷的含量。考察不同品牌色谱仪、色谱柱、检测波长、柱温、流速下各相对校正因子的变化，评价各校正因子的耐用性。分别采用外标法和所建立的一测多评法测定23批洋常春藤及30批提取物中4个皂苷类成分含量并比较两者的差异，以评价"一测多评"法的准确性。结果：23批洋常春藤中α-常春藤素、常春藤皂苷B、常春藤萜苷D的"一测多评"法测得含量与外标法结果之比分别为（100.56±0.29）%、（99.37±0.32）%、（99.52±0.18）%，30批提取物中相应成分2种方法的比值分别为（100.86±0.87）%、（99.84±0.02）%、（99.84±0.03）%；药材及提取物中2种方法所对应上述皂苷成分的相对偏差RE分别在0.10%~3.28%、0.12%~1.53%、0.11%~0.83%之间；3个常春藤皂苷的相对校正因子在不同仪器、不同色谱柱、不同检测波长、不同柱温及不同流速下的RSD分别为1.1%~3.6%、1.6%~2.0%、0.47%~3.1%、1.5%~2.9%与0.55%~2.5%，耐用性良好。结论：本文所建常春藤皂苷成分同时定量的一测多评方法准确度高、重复性及耐用性好，可用于洋常春藤及其提取物质量的常规检验与快速分析。
Objective: To establish a method for simultaneously determination of the contents of four saponins(hederacoside C,α-hederin,hederacoside D,hederasaponin B)in Hedera helix L.and its extract by quantitative analysis of multi-components by single-marker(QAMS).Methods: Taking hederacoside C as the reference substance,the relative correction factors(RCF)of α-hederin,hederacoside D and hederasaponin B were determined for assay of the three saponins by QAMS.The variance of the three RCFs caused by different instruments,chromatographic columns,detection wavelengths,column temperatures and flow rates was investigated to evaluate the durability of the factors.The contents of the three saponins in 30 batches of H.helix.and the corresponding extracts were determined by both external standard method and QAMS,respectively.The results of the two methods were compared to evaluate the accuracy of QAMS.Results: The ratio of the contents of the three saponins determined by QAMS to that by external standard method in 23 batches of the leaves of H helix and 30 batches of ivy extracts was(100.56±0.29)%,(99.37±0.32)%,(99.52±0.18)% and(100.86±0.87)%,(99.84±0.02)% and(99.84±0.03)%,respectively;the relative error of the corresponding saponins determined by two methods in H.helix and its extracts was in the range of 0.10%-3.28%,0.12%-1.53% and 0.11%-0.83%,respectively.And the three RCFs were consistent at different instruments,chromatographic columns,detection wavelengths,column temperatures and flow rates with the RSD within 1.1%-3.6%,1.6%-2.0%,0.47%-3.1%,1.5%-2.9% and 0.55%-2.5%,respectively.Conclusions: The established QAMS method has high accuracy and better repeatability and durability,it could be applied in routine and fast determination of main saponins in H.helix and its extract.
