HPLC法同时测定壮药玉叶金花中9个成分的含量

Objective:To establish an HPLC method for the simultaneous determination of nine components,including shanzhiside methyl ester,mussaenoside,8-O-acetyl shanzhiside methyl ester,neochlorogenic acid,chlorogenic acid,cyrptochlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid,in Zhuang medicine Mussaenda pubescens Ait. f. Methods:HPLC analysis was performed on a CAPCELL MGⅡC₁₈ column (4.6 mm×250 mm,5 μm). The mobile phase was acetonitrile-0.1% phosphoric acid with gradient elution. The flow rate was 1.0 mL·min^-1,the detection wavelength was 254 nm,the column temperature was 35℃ and the injection volume was 10 μL. Results:The standard curve revealed a good linear relationship over the ranges of 0.151-189.2 μg·mL^-1 for neochlorogenic acid,0.768-960.4 μg·mL^-1 for shanzhiside methyl ester,0.775-968.7 μg·mL^-1 for chlorogenic acid,0.886-1107 μg·mL^-1 for cyrptochlorogenic acid,0.734-917.0 μg·mL^-1 for mussaenoside,0.795-993.5 μg·mL^-1 for 8-O-acetyl shanzhiside methyl ester,0.872-1090 μg·mL^-1 for 3,4-O-dicaffeoylquinic,0.788-985.3 μg·mL^-1 for 3,5-O-dicaffeoylquinic and 0.767-958.9 μg·mL^-1 for 4,5-O-dicaffeoylquinic,respectively. Average recoveries,precision,reproducibility and stability were in accord with regulation. The contents of 9 components were 0.30-1.57 mg·g^-1 for neochlorogenic acid,0.34-5.20 mg·g^-1 for shanzhiside methyl ester,0.34-5.84 mg·g^-1 for chlorogenic acid,0.32-2.26 mg·g^-1 for cyrptochlorogenic acid,0.05-21.63 mg·g^-1 for mussaenoside,0.07-18.93 mg·g^-1 for 8-O-acetyl shanzhiside methyl ester,1.02-4.21 mg·g^-1 for 3,4-O-dicaffeoylquinic,0.39-5.77 mg·g^-1 for 3,5-O-dicaffeoylquinic and 1.38-5.25 mg·g^-1 for 4,5-O-dicaffeoylquinic,respectively. Conclusion:The developed method is suitable for the quantitative analysis of nine components in Mussaenda pubescens Ait. f.