气相色谱法同时测定苏合香丸中龙脑、丁香酚及麝香酮的含量

目的：建立同时测定苏合香丸中龙脑、丁香酚及麝香酮的含量的气相色谱法。方法：气相色谱条件：DB-5毛细管色谱柱（30 m×0.25 mm，0.25 μm），氢火焰离子化检测器（FID）；进样口温度200℃；检测器温度300℃；柱温以90℃为起始温度，以3℃·min^-1升温至140℃，保持3 min，以5℃·min^-1升温至280℃，保持15 min；载气为氮气，流速为1.5 mL·min^-1；进样方式为分流进样，分流比20：1；进样量为1 μL。结果：龙脑、丁香酚、麝香酮的浓度分别在0.035~3.5 mg·mL^-1（r=0.999 7），0.02~2.1 mg·mL^-1（r=0.999 8），0.016~1.56 mg·mL^-1（r=0.999 7）的范围内呈良好线性关系；平均回收率（n=9）分别为96.8%~98.7%，97.3%~100.3%，98.8%~100.4%。结论：该方法完善了现行标准，可高效、准确地同时测定苏合香丸中龙脑、丁香酚、麝香酮3个成分的含量。

Objective:To establish a GC method for the simultaneous determination of borneol,eugenol and muscone in Suhexiang pills.Methods:Chromatographic conditions:Capillary column DB-5(30 m×0.25 mm,0.25 μm)was used;The detector was FID;Inlet temperature was 200℃;The detector temperature was 300℃.The column temperature was programmed to start at 90℃,rise to 140℃ at a rate of 3℃·min^-1,and then rise to 280℃ at a rate of 5℃·min^-1,maintained for 15 min;Nitrogen(1.5 mL·min^-1)was used as the carrying gas,and the split ratio was 20:1;the injection volume was 1 μL.Results:the linear ranges of borneol,eugenol and muscone acetate were 0.035-3.5 mg·mL^-1(r=0.999 7),0.02-2.1 mg·mL^-1(r=0.999 8),0.016-1.56 mg·mL^-1(r=0.999 7),respectively.Their average recovery rates(n=9)were 96.8%-98.7%,97.3%-100.3%,98.8%-100.4%,respectively.Conclusion:The established method has improved the existing standards and it was efficient and accurate for the determination of borneol,eugenol and muscone in Suhexiang pills.