UPLC-MS/MS法同时测定蓝芩口服液中鸟苷、腺苷和胞苷的含量

目的：建立UPLC-MS/MS方法同时测定蓝芩口服液中鸟苷、腺苷和胞苷的含量。方法：采用ZORBAX SB C₁₈（2.1 mm×150 mm，5 μm）色谱柱，以甲醇-0.1%甲酸水溶液为流动相，梯度洗脱，流速0.2 mL·min^-1；质谱采用电喷雾（ESI）离子源，多反应监测模式（MRM）进行正离子扫描。结果：鸟苷、腺苷和胞苷质量浓度分别在10~100、15~150和20~200 ng·mL^-1范围内线性关系良好，平均加样回收率（n=3）分别为98.4%~102.3%、97.2%~99.6%和100.4%~103.6%，RSD分别为1.4%~2.8%、0.67%~2.2%和1.4%~2.5%。3批样品中鸟苷、腺苷和胞苷的含量范围分别为28.05~38.37、87.40~88.92和47.66~51.33 μg·mL^-1。结论：该方法适用于蓝芩口服液的质量控制。

Objective: To develop a UPLC-MS/MS method for simultaneous determination of guanosine,adenosine and cytidine in Lanqinoral liquid.Methods: Gradient elution was carried out with mobile phase consisting of methanol and 0.1% formic acid at a flow rate of 0.2 mL·min^-1.The separation was performed on a ZORBAX SB C₁₈(2.1 mm×150 mm,5 μm) column.The MS analysis was performed on a triple quadrupole mass spectrometer with an electrospray ionization(ESI) source operated in positive ion mode and multiple reaction monitoring(MRM).Results: Guanosine,adenosine and cytidine showed good linear relationship in the ranges of 10-100,15-150 and 20-200 ng·mL^-1,respectively. The average recoveries(n=3) of them were 98.4%-102.3%,97.2%-99.6% and 100.4%-103.6% with the RSD of 1.4%-2.8%,0.67%-2.2% and 1.4%-2.5%,respectively.The contents of them in batches of samples were in the range of 28.05-38.37,87.40-88.92 and 47.66-51.33 μg·mL^-1,respectively.Conclusion: The method can be used for quality control of Lanqin oral liquid.