UPLC-ELSD法测定12种保健食品中的牛磺酸

目的：建立保健食品中牛磺酸含量的测定方法。方法：采用超高效液相色谱-蒸发光散射（UPLC-ELSD）法测定保健食品中牛磺酸含量。色谱条件：采用BEH Amide（50 mm×2.1 mm，1.7 μm）色谱柱，梯度洗脱，流动相A为乙腈，流动相B为0.1%甲酸水，流速0.35mL·min^-1，柱温：40℃，ELSD漂移管温度40℃，ELSD气体压力150 kPa，增益值100。结果：牛磺酸浓度在0.10~2.0 mg·mL^-1范围内与峰面积呈良好线性关系（r=0.999 9），不同剂型保健食品中牛磺酸的回收率在98.5%~100.7%之间，RSD均小于1.3（n=6）。6批次液体制剂样品的测定结果分别为：38.60、8.702、0.501 0、5.152、5.085、3.821 mg·mL^-1；3批次固体制剂样品的测定结果分别为：6.703、4.560和15.81 g/100 g；2批次软胶囊样品的测定结果分别为：24.20、8.206 g/100 g；1批硬胶囊样品的测定结果为19.18%。结论：本法经方法学验证，可用于保健食品中牛磺酸的测定。

Objective: To establish a method for the determination of the contents of taurine in health food.Method: The content of taurine in health food was determined by ultra-performance liquid chromatography with evaporative light scattering detector.The chromatographic condition was as follows:BEH Amide(50 mm&#215;2.1 mm, 1.7 μm) was adopted, the mobile phase A was acetonitrile, the mobile phase B was water containing 0.1% formic acid at the flow rate of 0.35 mL&#183;min^-1 and the column temperature was 40℃, the drift tube temperature was 40℃, the gas pressure was 20 psi, the yield value was 100.Result: The calibration of taurine concentration was linear in the range of 0.10-2.0 mg&#183;mL^-1 (r=0.999 9), The average recovery was 98.5%-100.7% with RSD<1.3% (n=6).The contents of taurine in six batches of liquid samples were 38.60, 8.702, 0.501 0, 5.152, 5.085 and 3.821 mg&#183;mL^-1;The contents of taurine in three batches of solid samples were 6.703, 4.560 and 15.81 g/100 g;The contents of taurine in two batches of soft capsule samples were 24.20 and 8.206 g/100 g;The contents of taurine in one batch of hard capsule sample was 19.18%.Conclusion: It is proved by methodology validation that the method can be used for the determination of the contents of taurine in health food.