低分子量肝素核磁共振波谱法的定性鉴别方法研究

目的：建立低分子量肝素核磁共振波谱法（NMR）的定性鉴别方法。方法：一维¹³C-NMR实验是在装配5 mm BBO探头的Bruker Ascend-500核磁共振谱仪上采集。采样时间1.1秒；弛豫延长时间（D1）1秒；测量温度40℃ ；谱宽δ 236。结果：通过不同类型低分子量肝素标准品和市售产品的核磁共振一维碳谱精细结构信息比较发现，不同类型的低分子量肝素都可以通过碳谱特征信号进行区分。结论：核磁共振一维碳谱定性鉴别低分子量肝素类型的方法，专属性强，简单方便，是鉴别低分子量肝素较好的方法。

Objective: To establish an NMR method for the identification of low molecular weight heparin. Method: Carbon nuclear magnetic resonance(¹³C-NMR)spectra were collected by using a Bruker Ascend 500 spectrometer with 5 mm BBO probe. The measurement parameters setting were:acquisition time 1.1 s, relaxation delay(D1)1 s, temperature 40℃, and spectrum width δ 236. Result: By comparing the ¹³C-NMR spectra of low molecular weight reference standard and purchased heparin, there were some unique signals in the ¹³C-NMR spectra, which could be used to identify different types of low molecular weight heparin. Conclusion: 1D ¹³C-NMR spectroscopy is a specific, simple and convenient method for the identification of low molecular weight heparin.

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