目的：建立顶空-气相色谱法测定人参总皂苷中10种树脂残留成分的含量。方法：以10%的N，N-二甲基甲酰胺为提取溶剂，采用顶空进样，毛细管柱气相色谱测定树脂残留成分。柱温为程序升温（起始温度为60℃，保持1 min，以0.3℃·min-1升至62℃，再以10℃·min-1升至140℃，保持5 min）；进样口温度：220℃，检测器温度：250℃。FID检测器，载气为氮气，流速1 mL·min-1，分流比为10：1；顶空进样，顶空平衡温度90℃，平衡时间为30 min，定量环温度110℃，传输管温度130℃，进样量1 mL，低速振摇。结果： 10种有机溶剂残留物的峰面积与质浓度呈良好线性关系，正己烷、甲基环己烷、苯、甲苯、对二甲苯、间二甲苯、邻二甲苯、苯乙烯、1，2-二乙基苯和二乙烯苯的平均回收率分别为102.65%、100.96%、103.34%、104.69%、96.14%、96.83%、96.81%、103.78%、94.69%和102.64%，3批市售样品除检出残留物邻二甲苯（含量分别为1.51、1.18、1.48μg·g-1）外，其他9种残留成分未检出。结论：所建立的方法能够较好地对人参总皂苷中10种树脂残留物进行限度测定。
Objective: To develop a determination method of ten resin residues in total saponins of Panax Ginseng by headspace GC. Method: The sample was dissolved with 10% DMF and analyzed by headspace capillary gas chromatography. The temperature program for the column was as follows:initially heated at 60℃ for 1 min, rose to 62℃ at a speed of 0. 3℃·min-1; then rose to 140℃ at a speed of 10℃·min-1, lasting for 5 min. The inlet temperature was 220℃ and FID detector temperature was set at 250℃. The split ratio was 10:1 and the flow rate of the carrier gas(N2)was 1 mL·min-1. Headspace conditions were as follows:the temperature and the time of balance for the sample were 90℃ and 30 min; the temperature of quantitative loop and transfer lines was 110℃ and 130℃. The sample volume was 1 mL, with low-speed shaking. Result: Linear relations between peak area and concentration of ten residues were good, average recoveries of n-hexane, hexahydrotoluene, benzene, methylbenzene, p-xylene, m-xylene, o-xylene, styrene, 1, 2-diethylbenzene and divinylbenzene were 102. 65%, 100. 96%, 103. 34%, 104. 69%, 96. 14%, 96. 83%, 96. 81%, 103. 78%, 94. 69% and 102. 64%, respectively. O-xylene was detected in three batches of samples from market(the content was 1. 51, 1. 18 and 1. 48 μg·g-1, respectively), and the other nine residues were not found in the samples. Conclusion: The method was suitable for detecting ten resin residues in total saponins of Panax Ginseng.
