目的：以全时段双波长融合HPLC图谱定量为主要技术手段，对山绿茶药材进行质量控制方法的研究。方法：采用Poroshell SB-C18色谱柱（4.6 mm×100 mm，2.7 m），流动相为0.1%甲酸水溶液-乙腈，线性梯度洗脱，流速0.8 mL·min-1，柱温30℃，检测波长256 nm（芦丁、异槲皮苷）、328 nm（绿原酸、异绿原酸A、异绿原酸B、异绿原酸C），使用Matlab软件编程，对dif格式数据进行全时段双波长融合。结果：芦丁、异槲皮苷、绿原酸、异绿原酸A、异绿原酸B、异绿原酸C进样量分别在0.449~5.837、0.483~6.279、0.415~5.392、0.922~11.986、1.013~13.169、1.418~18.434 μg范围内呈良好的线性关系，平均加样回收率为98.3%~101.8%（RSD<3%，n=6）；10批样品中芦丁、异槲皮苷、绿原酸、异绿原酸A、异绿原酸B、异绿原酸C含量范围分别为17.69~94.43、12.72~49.43、13.41~66.48、52.90~148.70、49.82~241.10、62.49~183.63 mg·g-1。结论：该方法可以为山绿茶的多成分质量控制提供依据。
Objective: To establish the method for quality control of Ilex hainanensis Merr. based on the full time two-wavelength fusion of HPLC spectra.Methods: The separation was performed on Poroshell SB-C18 column(4. 6 mm×100 mm, 2. 7 m)with linear gradient elution of 0. 1% methanoic acid in water and acetonitrile. The flow rate was 0. 8 mL·min-1 and the temperature was 30℃. The detections of rutin and isoquercitrin were at 256 nm,those of chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C were at 328 nm. Matlab was used for full time dual wavelength fusion of dif date.Results: A good linearity was found in the range of 0. 449-5. 837 μg,0. 483-6. 279 μg,0. 415-5. 392 μg,0. 922-11. 986 μg,1. 013-13. 169 μg,1. 418-18. 434 μg for rutin, isoquercitrin, chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C, respectively. The average recovery was 98. 3%-101. 8%(RSD<3%, n=6). The contents of rutin, isoquercitrin, chlorogenic acid,isochlorogenic acid A,isochlorogenic acid B and isochlorogenic acid C in ten samples were in the rages of 17. 69-94. 43 mg·g-1, 12. 72-49. 43 mg·g-1, 13. 41-66. 48 mg·g-1, 52. 90-148. 70 mg·g-1, 49. 82-241. 10 mg·g-1, 62. 49-183. 63 mg·g-1, respectively.Conclusion: The established method is helpful to control the quality of Ilex hainanensis based on the multi-ingredient.
