目的：建立青钱柳叶中铅、镉、砷、汞、铜5种有害重金属元素的检测方法，并测定不同来源青钱柳叶中5种重金属的残留量。方法：采用全自动石墨炉消解仪对样品进行消解，利用原子吸收分光光度法测定青钱柳叶中的铅、镉、铜，利用原子荧光分光光度法测定青钱柳叶中的砷，采用压力罐消解法对样品进行消解，利用原子荧光分光光度法测定青钱柳叶中的汞，并对检测方法进行方法学考察。结果：铅、镉、砷、汞、铜5种重金属元素检测方法经方法学考察，结果合理有效，标准曲线的线性相关系数均大于0.996，平均加样回收率分别为89.6%、90.1%、92.2%、96.4%、92.1%。不同来源样品5种重金属元素含量范围分别为：0.974 3~4.058、0.054 4~0.276 4、0.321 2~0.768 5、0.085 36~0.146 2、5.259~16.68 mg·kg-1。结论：本研究采用的方法简单，准确，重复性好，可用于准确测定青钱柳叶中铅、镉、砷、汞、铜5种重金属的含量，并为青钱柳叶提供更为全面的质量控制方法。
Objective: To establish methods for determination of heavy metals including plumbum, cadmium, arsenic, mercury and cuprum in leaves of Cyclocarya paliurus so as to determine the residue of these five heavy metals in leaves of Cyclocarya paliurus from diferent areas. Methods: The samples were digested by automatic graphite furnace digestion instrument. Determination of plumbum(Pb), cadmium(Cd)and copper(Cu)was wnducted by atomic absorption spectrometry. Determination of arsenic(As)was conducted by atomic fluorescence spectrometry. Determination of mercury(Hg)by using high pressure digestion and atomic fluorescence spectrometry. And the methods were validated. Results: The methods of Pb, Cd, As, Hg and Cu were reasonable and effective by validation. The standard curves were linear and the linear correlation coefficients were greater than 0.996. The average recoveries were 89.6%, 90.1%, 92.2%, 96.4%, 92.1%, respectively. The contents of 5 heavy metals in different areas were 0.974 3-4.058 mg·kg-1, 0.054 4-0.277 6 mg·kg-1, 0.321 2-0.768 5 mg·kg-1, 0.085 36-0.146 2 mg·kg-1, 5.259~16.68 mg·kg-1, respectively. Conclusion: These established methods in the research are simple, accuracy, reproducible and can be used for the determination of the five heavy metals in leaves of CycLocarya paliurus, and provide more comprehensive quality control methods for leaves of Cyclocarya paliarus.
