高效液相色谱联用电喷雾检测器分析不同植物中棉子糖系列寡糖

目的：应用高效液相色谱联用电喷雾检测器（HPLC-CAD）分析不同植物中棉子糖系列寡糖（RFOs）。方法：采用微波辅助提取法制备RFOs提取物，再应用HPLC-CAD进行定性、定量分析。色谱条件：采用氨基色谱柱（4.6 mm×250 mm，3.5 μm），流动相为水（A）-乙腈（B），梯度洗脱（0~20 min，75% B→45% B，20-22 min，45% B→75% B），流速为1 mL·min^-1，柱温为30℃。外标一点法定量。结果：供试品溶液在48 h内稳定性较好，RSD小于2.8%；低、中、高浓度供试品溶液的重复性RSD分别小于3.6%、3.8%和3.1%。RFOs在豆科植物种子中分布广泛，其在水苏（总量426.19 mg·g^-1）和生地黄（总量373.40 mg·g^-1）中含量较高，而在车前草（总量1.08 mg·g^-1）中含量极低。结论：水苏和地黄有潜力成为开发RFOs新资源。

Objective:To determine raffinose family oligosaccharides (RFOs) in different plants using high performance liquid chromatography coupled with charged aerosol detector (HPLC-CAD).Methods:RFOs were extracted from selected plants by using microwave assisted extraction.Then the extract was analyzed using HPLC-CAD.The separation was performed on an amide column (4.6 mm×250 mm,3.5 μm).The mobile phase consists of water (A) and acetonitrile (B) with gradient elution(0-20 min,75%B→45%B,20-22 min,45%B→75%B).The flow rate and the column temperature were 1.0 mL·min^-1 and 30℃,respectively.Single point external standard method was used for quantification of RFOs.Results:Samples were stable in 48 h with RSD less than 2.83%.The repeatability presented as RSDs of sample solution in low,middle and high levels were 3.6%,3.8% and 3.1%,respectively.RFOs were widely distributed in different seeds of edible plants from Leguminosae family,and the high amount of RFOs was found in Stachys japonica (total content 426.19 mg·g^-1) and Rehmannia glutinosa (total content 426.19 mg·g^-1),but only a small amount of RFOs was found in Plantago asiatica (total content 1.08 mg·g^-1).Conclusion:Rehmannia glutinosa and Stachys japonica might be new potential sources of RFOs.