RP-HPLC同时测定地黄饮子中松果菊苷、毛蕊花糖苷、马钱苷的含量

目的：建立反相高效液相色谱法（RP-HPLC法）同时测定地黄饮子中松果菊苷、毛蕊花糖苷、马钱苷的含量。方法：采用Kromasil C₁₈（150 mm×4.6 mm，5 μm）色谱柱，以甲醇-0.01%磷酸水溶液为流动相进行梯度洗脱，流速1.0 mL·min^-1，检测波长为334 nm（松果菊苷及毛蕊花糖苷）和240 nm（马钱苷），进样量20 μL。结果：松果菊苷、毛蕊花糖苷、马钱苷线性范围分别为3.37~67.40 μg·mL^-1（r=0.999 2）、3.31~66.20 μg·mL^-1（r=0.999 3）和5.76~115.2 μg·mL^-1（r=0.999 6），平均加样回收率（n=6）分别为97.2%、98.1%和98.5%。6批样品中松果菊苷、毛蕊花糖苷、马钱苷含量分别为73.25~81.36、51.54~60.28、14.77~17.08 μg·mL^-1。结论：本方法可用于地黄饮子中松果菊苷、毛蕊花糖苷及马钱苷的含量测定。

Objective: To establish an RP-HPLC method for simultaneous determination of echinacoside,verbascoside and loganin in Dihuangyinzi. Methods: A Kromasil C₁₈ column(150 mm×4.6 mm,5 μm)was adopted as analytical column. The mobile phase consisted of methanol-0.01% phosphoric acid with gradient elution at a flow rate of 1.0 mL·min^-1,the detection wavelengths were 334 nm for echinacoside and verbascoside,240 nm for loganin,and the injection volume was 20 μL. Results: The linear response ranges observed were 3.37-67.40 μg·mL^-1(r=0.999 2),3.31-66.20 μg·mL^-1(r=0.999 3)and 5.76-115.2 μg·mL^-1(r=0.999 6),respectively;the average recoveries(n=6)were 97.2%,98.1% and 98.5%,respectively. The contents of echinacoside,verbascoside,loganin in six batches of samples were 73.25-81.36,51.54-60.28 and 14.77-17.08 μg·mL^-1,respectively. Conclusion: The method can be used for simultaneous determination of sechinacoside,verbascoside and loganin in Dihuangyinzi.

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