HPLC-DAD同时测定左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸含量

目的：建立同时测定左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸5种化学成分含量的分析方法。方法：采用HPLC-DAD多波长切换-梯度洗脱技术。色谱柱：Eclipse XDB-C₁₈（4.6 mm×250mm，5 μm）；流动相：甲醇-1%醋酸水溶液，梯度洗脱；流速：1.0 mL·min^-1；分别以各成分的最大吸收波长为检测波长；柱温：30℃。结果：左归丸药液中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸5种成分均具有良好的线性关系（r≥0.999），平均加样回收率为97.76%～101.8%（RSD<3%，n=6）。3批样品中没食子酸、原儿茶酸、绿原酸、马钱苷、阿魏酸含量分别为0.489 0～0.500 6、0.104 4～0.108 4、0.048 56～0.050 66、1.042～1.061、5.287×10^-3～5.369×10^-3 mg·mL^-1结论：该方法经方法学验证，可用于左归丸药液及其制剂的质量控制。

Objective: To establish a method for simultaneous determination of gallic acid,protocatechuic acid,chlorogenic acid,loganin and ferulic acid contents in herb liquor of Zuogui pills.Methods: HPLC-DAD and the gradient elution wavelength shifts technique were used in the study.The column was Eclipse XDB-C₁₈ (4.6 mm×250 mm,5 μm);the mobile phase was methanol-1% acetic acid solution at the flow rate of 1 mL·min^-1, the detection wavelength was the maximum absorption wavelength of each ingredient;the oven temperature was 30℃. Results: The contents of all 5 indicative components in herb liquor of Zuogui Pill had a good linearity(r≥0.999). The average recoveries were 97.76%-101.8%(RSD<3%,n=6).The contents of gallic acid,protocatechuic acid,chlorogenic acid,loganin and ferulic acid in 3 samples were 0.489 0-0.500 6 mg·mL^-1,0.104 4-0.108 4 mg·mL^-1,0.048 56-0.050 66 mg·mL^-1,1.042-1.061 mg·mL^-1,5.287×10^-3-5.369×10^-3 mg·mL^-1, respectively.Conclusion: This method has been validated,so it can be used to control the quality of herb liquor of Zuogui Pill and its preparation.