HPLC法测定不同产地通关藤中通关藤苷G和I的含量

目的:建立通关藤中通关藤苷G和I的含量测定方法,并比较不同产地通关藤中两者的含量。方法:应用HPLC法,采用Ecosil C₁₈(4.6 mm×150 mm,5μm)色谱柱,以乙腈-水(48:52)为流动相,流速1.0 mL·min^-1,检测波长230 nm,柱温30℃。结果:通关藤中通关藤苷G和I分别在0.4124~4.1240μg(r=0.9995)和0.1596~1.5960μg(r=0.9997)范围内线性关系良好,平均回收率分别为99.5%(RSD=2.4%)和100.0%(RSD=2.4%),不同产地药材中通关藤苷G和I含量范围分别为0.472%~0.882%和0.118%~0.362%。结论:本法准确度,重复性好,专属性强,可控制药材的质量;且不同产地药材中通关藤苷G和I 2种成分含量有一定差异性,其中云南丽江含量最高,可指导临床用药。

Objective: To develop an HPLC method for determination of the proportion of tenacissosides G and I in the caulis of Marsdenia tenacissima(Roxb.) Wight et Arn.from different origins, so as to select the best origins of Marsdenia tenacissima(Roxb.) Wight et Arn.Methods: An HPLC method was established using Ecosil C₁₈ column(4.6 mm×150 mm, 5 μm).The mobile phase was acetonitrile-water(48:52), the flow rate was 1.0 mL·min^-1, the detection wavelength was 230 nm, and the column temperature was 30℃.Results: The calibration curves of tenacissosides G and I were linear in the ranges of 0.4124-4.1240 μg(r=0.999 5) and 0.159 6-1.596 0 μg (r=0.999 7), respectively.The average recoveries of tenacissosides G and I were 99.5%(RSD=2.4%) and 100.0%(RSD=2.4%), respectively.The contents of tenacissosides G and I in the caulis of M.tenacissima from different origins were 0.472%-0.882% and 0.118%-0.362%.Conclusion: The established method, which is accurate, reproducible and precise, can be applied to the quality control of tenacissosides G and I in the caulis of M.tenocissimae. The content of two ingredients in herbs of different origins are different, among which the highest in samples from Lijiang and lowest from Guiyang;these results will benefit the clinical is found in.