酒女贞子HPLC指纹图谱的建立及多成分含量的测定

目的:建立酒女贞子饮片的HPLC指纹图谱及建立指标成分含量测定的方法,为酒女贞子饮片的质量控制提供参考。方法:采用ODS C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长230 nm,建立酒女贞子HPLC指纹图谱,并对红景天苷、齐墩果酸、木犀草素及特女贞苷指标成分的含量测定方法进行方法学考察。结果:红景天苷、齐墩果酸、木犀草素及特女贞苷线性范围分别为0.464~2.320、0.204~4.080、0.041~0.205和0.620~3.100 μg,线性关系良好(r>0.9998)。使用外标法测定红景天苷、齐墩果酸、木犀草素和特女贞苷含量分别在2.648~4.681、5.082~11.770、0.059~0.107和5.158~12.25 mg·g^-1范围内。取10个收集地酒女贞子饮片建立HPLC指纹图谱,以木犀草素峰为参比峰,样品相似度与对照图谱相似度均≥0.95,说明各地出售的酒女贞子饮片质量相对稳定。结论:该方法简便快速,分离效果好,重复性好,可为规范及评价酒女贞子质量及同属植物的研究提供参考。

Objective:To establish an HPLC fingerprint method for the determination of ingredients of wine-fried Ligustri Lucidi Fructus,so as to provide a reference for the quality control.Methods:ODS C₁₈(250 mm×4.6 mm,5μm)column was adopted,the mobile phase consisted of acetonitrile-water with gradient elution at the flow rate of 1.0 mL·min^-1,and the detection wavelength was 230 nm;the establishment of wine-fried Ligustri Lucidi Fructus HPLC fingerprint,and salidroside,determination method of oleanolic acid,luteolin and specnuezhenide index components were studied methodologically.Results:Good linear relationship were observed for Salidroside,oleanolic acid,luteolin and specnuezhenide in the ranges of 0.464-2.320,0.204-4.080, 0.041-0.205 and 0.620-3.100 μg,respectively(r>0.9998).The content ranges of alidroside,oleanolic acid,luteolin and specnuezhenide were 2.648-4.681,5.082-11.770,0.059-0.107 and 5.158-12.25 mg·g-by external standard method of determination.Different wine-fried Ligustri Lucidi Fructus were collected from 10 areas to establish HPLC fingerprint,and the luteolin was employed as a reference.The similarity of sample and standard drug is equal to 0.95 or higher,which indicated that the quality of wine-fried Ligustri Lucidi Fructus is relatively stable.Conclusion:The established method is simple,rapid,in good separation and reproducibility, and thus can provide a reference for the standard and evaluation of quality of wine-fried Ligustri Lucidi Fructus and congeneric plant.