2,4-二硝基氟苯柱前衍生化HPLC法测定保健食品中牛磺酸含量

目的:建立保健食品中牛磺酸含量的测定方法。方法:采用2, 4-二硝基氟苯进行柱前衍生化,HPLC法测定保健食品中牛磺酸含量。色谱条件:采用HyperClone BDS C₁₈(250 mm×4.6 mm, 5μm)色谱柱,以乙腈-0.05 mol·L^-1磷酸二氢钠溶液(用5%氢氧化钠溶液调pH至6.5)(15:85)为流动相,流速1.0 mL·min^-1,检测波长360 nm,柱温30℃。结果:牛磺酸质量浓度在4.684~46.840μg·mL^-1范围内与峰面积呈良好线性关系(r=0.9998),不同剂型保健品中牛磺酸的平均回收率在96.5%~98.4%之间,RSD均小于2.1%(n=6)。3批次软胶囊样品测定结果分别为246.0、230.0、235.9 mg·g^-1; 4批次固体样品测定结果分别为214.7、25.92、123.9、58.70 mg·g^-1; 9批次液体样品测定结果分别为2189、3378、491.5、1958、423.4、439.4、493.7、89241、6321 mg·L^-1。结论:本法经方法学验证,可用于保健食品中牛磺酸的测定。

Objective:To establish a method for determination of taurine in health food. Methods:After pre-column derivatization with 2, 4-dinitrofluorobenzene, the content of taurine in health food was determined by HPLC. The chromatographic condition was follows. HyperClone BDS C₁₈ column(250 mm×4.6 mm,5μm)was adopted,the mobile phase consisted of acetonitrile-0.05 mol·L^-1 sodium dihydrogen phosphate solution(pH adjusted to 6.5 with 5% sodium hydroxide)(15:85)at the flow rate of 1.0 mL·min^-1,the detection wavelength was set at 360 nm and the column temperature was 30℃. Results:The calibration curve of taurine concentration was linear in the range of 4.684-46.840μg·mL^-1(r=0.9998). The average recovery was 96.5%-98.4% with RSD<2.1%(n=6). The contents of taurine in three batches of soft capsule samples were 246.0 mg·g^-1, 230.0 mg·g^-1 and 235.9 mg·g^-1; the contents of taurine in four batches of solid samples were 214.7 mg·g^-1, 25.92 mg·g^-1, 123.9 mg·g^-1 and 58.70 mg·g^-1;the contents of taurine in nine batches of liquid samples were 2189 mg·L^-1, 3378 mg·L^-1, 491.5 mg·L^-1, 1958 mg·L^-1, 423.4 mg·L^-1, 439.4 mg·L^-1, 493.7 mg·L^-1, 8924 mg·L^-1 and 6321 mg·L^-1.Conclusion:It is proved by methodology validation that the method can be used for determination of taurine in health food.