HPLC法同时测定苍术-玄参药对中4种活性成分的含量

目的:建立高效液相色谱法同时测定苍术-玄参药对中哈巴苷、安格洛苷C、哈巴俄苷、苍术素4种有效成分的含量。方法:采用Phenomenex luna C₁₈色谱柱(250 mm×4.6 mm, 5.0μm),以乙腈(A)-水溶液(B)为流动相,线性梯度洗脱(0~5 min, 10%A → 33%A;5~8 min, 33%A → 50%A;8~18 min, 50%A → 60%A; 18~20 min, 60%A → 80%A; 20~35 min, 80%A → 70%A; 35~40 min,70%A → 10%A),流速1.0 mL·min^-1,检测波长210 nm和280 nm,柱温25℃。结果:哈巴苷、安格洛苷C、哈巴俄苷、苍术素质量浓度分别在0.024~0.48、0.029~0.58、0.027~0.54和0.026~0.52 mg·mL^-1范围内呈良好线性关系;平均回收率(n=9)分别为100.1%、99.5%、99.1%、99.3%。3批苍术-玄参药对中哈巴苷、安格洛苷C、哈巴俄苷、苍术素含量范围分别为5.16%~6.95%、0.352%~0.517%、0.041%~0.068%和0.053%~0.071%。结论:该方法结果可靠准确,操作简便,可用于苍术-玄参药对中4种有效成分的含量测定。

Objective:To establish a method for simultaneous determination of harpagide, angoroside C, harpagoside, atractylodin in herbal pair rhizome of Atractylodes lancea-Radix Scrophulariae. Methods:A Phenomenex Luna C 18 column(250 mm×4.6 mm,5μm)was adopted. The mobile phase consisted of acetonitrile(A)-water(B)with linear gradient elution(0-5 min,10%A → 33%A; 5-8 min,33%A → 50%A; 8-18 min, 50%A → 60%A; 18-20 min, 60%A → 80%A; 20-35 min, 80%A → 70%A; 35-40 min, 70%A → 10%A). The flow rate was 1.0 mL·min^-1. The detection wavelength was 210 nm and 280 nm. The column temperature was controlled at 25℃. Results:Harpagide, angoroside C, harpagoside, atractylodin exhibited good linearity among the ranges of 0.024-0.48 mg·mL^-1, 0.029-0.58 mg·mL^-1, 0.027-0.54 mg·mL^-1, and 0.026-0.52 mg·mL-1, respectively; the average recoveries(n=9)were 100.1%, 99.5%, 99.1%, and 99.3%,respectively. The content ranges of harpagide, angoroside C, harpagoside, atractylodin in herbal pair rhizome of Atractylodes lancea-Radix Scrophulariae were 5.16%-6.95%, 0.352%-0.517%, 0.041%-0.068%, and 0.053%-0.071%, respectively. Conclusion:The established method is simple and accurate, and thus can be applied to determinate four active ingredients in herbal pair rhizome of Atractylodes lancea-Radix Scrophulariae.