柴胡汤剂中酰化柴胡皂苷a、b₂的含量测定

目的:建立柴胡汤剂中酰化柴胡皂苷a、b₂的含量测定方法。方法:采用单因素分析和正交实验设计法,选择pH、超声时间、超声功率为因素各取3水平,按L₉(3⁴)正交表进行实验,优化汤剂中酰化柴胡皂苷a、b₂的水解方法。采用HPLC法测定柴胡皂苷的含量。色谱条件:采用WondaSil C₁₈色谱柱(4.6 mm×250 mm, 5μm),以甲醇-水(53:47)为流动相,流速1.0 mL·min^-1,检测波长210 nm(检测酰化柴胡皂苷a)、250 nm(检测酰化柴胡皂苷b₂)。结果:优化的酰化柴胡皂苷水解方法为pH为10.0,超声(功率为500 W,频率40 kHz)40 min;酰化柴胡皂苷a含量占汤剂中柴胡皂苷a含量的29%~41%,而酰化柴胡皂苷b₂的含量占汤剂中柴胡皂苷b₂的90%~115%。结论:该方法简便可靠,可用于汤剂中酰化柴胡皂苷a、b₂的含量测定,并可为完善柴胡汤剂质量评价提供参考。

Objective:To establish the determination method of acyl-saikosaponins a and b₂ in Radix Bupleuri decoction. Methods:Single factor analysis and orthogonal experimental design were used to optimize the acylsaikosaponin hydrolysis parameters such as pH, time of ultrasound-assisted hydrolysis and ultrasound power by L₉(3⁴)orthogonal test. An HPLC method was employed to determine the saikosaponins contents, which was carried out on a WondaSil C 18 column(4.6 mm×250 mm, 5μm)with the mobile phase of methanol-water(53:47)with the flow rate at 1.0 mL·min^-1 and the wavelengths of 210 nm for acyl-saikosaponin a and 250 nm for acyl-saikosaponin b₂. Results:The optimized parameters of acyl-saikosaponin were listed as follow:the pH value was 10.0, the time of ultrasound-assisted hydrolysis was 40 min with ultrasound power of 500 W and ultrasound frequency of 40 kHz. In Radix Bupleuri decoction, the content of acyl-saikosaponin a accounted for 29%-41% of saikosqaponin a and acyl-saikosaponin b₂ accounted for 90%-115% of saikosaponin b₂. Conclusion:This method is simple and reliable,and can be used for the determination of acyl-saikosaponins a and b 2 in Radix Bupleuri decoction and gives clues for the better quality assessment of Radix Bupleuri decoction.